Intraamygdaloid microinjection of RFamide-related peptide-3 decreases food intake in rats

Anita Kovács, Kristóf László, Rita Gálosi, Tamás Ollmann, László Péczely, Olga Zagoracz, Noémi Bencze, László Lénárd

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Some members of the RFamide peptide family are associated with feeding in rodents. For example, neuropeptide FF and prolactin-releasing peptide cause anorexigenic, while 26RFa and QRFP result in orexigenic effects. I.c.v. microinjection of RFamide-related peptide-3 (RFRP-3) facilitates feeding. Feeding related effects of RFRP-3, however, have not been studied after direct brain microinjections in rats. The central part of amygdala (CeA) is essentially involved in the regulation of feeding and body weight. RFRP-3 positive nerve cells were detected in the rat hypothalamus and RFRP-3 immunoreactive fibers were identified in the CeA. RFRP analogs bind with relatively high affinity to the NPFF1 and NPFF2 receptors (NPFF-R). RFRP-3 has potent activity for NPFF-1 that is expressed in the CeA. To evaluate the role of RFRP-3 in feeding regulation rats were microinjected with different doses of RFRP-3 and their food intake were quantified over a 60. min period. Liquid food intake of male Wistar rats was measured after bilateral intraamygdalar administration of RFRP-3 (25, 50, 100 or 200. ng/side, RFRP-3 dissolved in 0.15. M sterile NaCl/0.4. μl, respectively). The 50. ng and 100. ng doses of RFRP-3 microinjections resulted in significant decrease of food intake. Twenty-five and 200. ng had no effect. Food intake decreasing effect of RFRP-3 was eliminated by NPFF-R antagonist RF9 pretreatment. In open-field test effective doses of RFRP-3 did not modify spontaneous locomotor activity and general behavior of animals did not change. Our results are the first reporting that RFRP-3 injected to the CeA resulted in a decrease of liquid food consumption. This is a receptor-linked effect because it was eliminated by NPFF-R antagonist.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalBrain Research Bulletin
Publication statusPublished - Aug 2014



  • Amygdala
  • Food intake
  • General activity
  • NPFF-R antagonist RF9
  • Neuropeptide RFRP-3

ASJC Scopus subject areas

  • Neuroscience(all)

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