Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila

Gábor Pápai, Orbán Komonyi, Zsolt Tóth, Tibor Pankotai, Selen Muratoglu, A. Udvardy, I. Boros

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

PIMT, a transcriptional coactivator which interacts with and enhances nuclear receptor coactivator PRIP function, was identified recently in mammalian cells and suggested to function as a link between two major multiprotein complexes anchored by CBP/p300 and PBP. Here we describe that the gene of the Drosophila homologue of PIMT, designated as Dtl, is closely associated and has an overlapping promoter with a gene encoding another transcriptional coactivator, ADA2a, which in turn participates in GCN5 HAT-containing complexes. Ada2a also produces an RNA polII subunit, RPB4, via alternative splicing; consequently, an overlapping regulatory region serves for the production of three proteins, each involved in transcription. By studying expression of reporter gene fusions in tissue culture cells and transgenic animals we have demonstrated that the regulatory regions of Ada2a/Rpb4 and Dtl overlap and the Dtl promoter is partly within the Ada2a/Rpb4 coding region. The shared regulatory region contains a DRE element, binding site of DREF, the protein factor involved in the regulation of a number of genes which play a role in DNA replication and cell proliferation. Despite the perfectly symmetrical DRE, DREF seems to have a more decisive role in Ada2a/Rpb4 transcription than in the transcription of Dtl.

Original languageEnglish
Pages (from-to)13-23
Number of pages11
JournalGene
Volume348
Issue number1-2 SUPPL.
DOIs
Publication statusPublished - Mar 28 2005

Fingerprint

Nucleic Acid Regulatory Sequences
Drosophila
Nuclear Receptor Coactivators
Genes
Multiprotein Complexes
Genetically Modified Animals
Gene Fusion
Alternative Splicing
DNA Replication
Reporter Genes
Proteins
Cell Culture Techniques
Binding Sites
Cell Proliferation
RNA

Keywords

  • ADA2a
  • DRE motif
  • Drosophila promoter
  • PIMT

ASJC Scopus subject areas

  • Genetics

Cite this

Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila. / Pápai, Gábor; Komonyi, Orbán; Tóth, Zsolt; Pankotai, Tibor; Muratoglu, Selen; Udvardy, A.; Boros, I.

In: Gene, Vol. 348, No. 1-2 SUPPL., 28.03.2005, p. 13-23.

Research output: Contribution to journalArticle

Pápai, Gábor ; Komonyi, Orbán ; Tóth, Zsolt ; Pankotai, Tibor ; Muratoglu, Selen ; Udvardy, A. ; Boros, I. / Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila. In: Gene. 2005 ; Vol. 348, No. 1-2 SUPPL. pp. 13-23.
@article{3ea26c52fdc44e9284514cbe1062dcd1,
title = "Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila",
abstract = "PIMT, a transcriptional coactivator which interacts with and enhances nuclear receptor coactivator PRIP function, was identified recently in mammalian cells and suggested to function as a link between two major multiprotein complexes anchored by CBP/p300 and PBP. Here we describe that the gene of the Drosophila homologue of PIMT, designated as Dtl, is closely associated and has an overlapping promoter with a gene encoding another transcriptional coactivator, ADA2a, which in turn participates in GCN5 HAT-containing complexes. Ada2a also produces an RNA polII subunit, RPB4, via alternative splicing; consequently, an overlapping regulatory region serves for the production of three proteins, each involved in transcription. By studying expression of reporter gene fusions in tissue culture cells and transgenic animals we have demonstrated that the regulatory regions of Ada2a/Rpb4 and Dtl overlap and the Dtl promoter is partly within the Ada2a/Rpb4 coding region. The shared regulatory region contains a DRE element, binding site of DREF, the protein factor involved in the regulation of a number of genes which play a role in DNA replication and cell proliferation. Despite the perfectly symmetrical DRE, DREF seems to have a more decisive role in Ada2a/Rpb4 transcription than in the transcription of Dtl.",
keywords = "ADA2a, DRE motif, Drosophila promoter, PIMT",
author = "G{\'a}bor P{\'a}pai and Orb{\'a}n Komonyi and Zsolt T{\'o}th and Tibor Pankotai and Selen Muratoglu and A. Udvardy and I. Boros",
year = "2005",
month = "3",
day = "28",
doi = "10.1016/j.gene.2005.01.006",
language = "English",
volume = "348",
pages = "13--23",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2 SUPPL.",

}

TY - JOUR

T1 - Intimate relationship between the genes of two transcriptional coactivators, ADA2a and PIMT, of Drosophila

AU - Pápai, Gábor

AU - Komonyi, Orbán

AU - Tóth, Zsolt

AU - Pankotai, Tibor

AU - Muratoglu, Selen

AU - Udvardy, A.

AU - Boros, I.

PY - 2005/3/28

Y1 - 2005/3/28

N2 - PIMT, a transcriptional coactivator which interacts with and enhances nuclear receptor coactivator PRIP function, was identified recently in mammalian cells and suggested to function as a link between two major multiprotein complexes anchored by CBP/p300 and PBP. Here we describe that the gene of the Drosophila homologue of PIMT, designated as Dtl, is closely associated and has an overlapping promoter with a gene encoding another transcriptional coactivator, ADA2a, which in turn participates in GCN5 HAT-containing complexes. Ada2a also produces an RNA polII subunit, RPB4, via alternative splicing; consequently, an overlapping regulatory region serves for the production of three proteins, each involved in transcription. By studying expression of reporter gene fusions in tissue culture cells and transgenic animals we have demonstrated that the regulatory regions of Ada2a/Rpb4 and Dtl overlap and the Dtl promoter is partly within the Ada2a/Rpb4 coding region. The shared regulatory region contains a DRE element, binding site of DREF, the protein factor involved in the regulation of a number of genes which play a role in DNA replication and cell proliferation. Despite the perfectly symmetrical DRE, DREF seems to have a more decisive role in Ada2a/Rpb4 transcription than in the transcription of Dtl.

AB - PIMT, a transcriptional coactivator which interacts with and enhances nuclear receptor coactivator PRIP function, was identified recently in mammalian cells and suggested to function as a link between two major multiprotein complexes anchored by CBP/p300 and PBP. Here we describe that the gene of the Drosophila homologue of PIMT, designated as Dtl, is closely associated and has an overlapping promoter with a gene encoding another transcriptional coactivator, ADA2a, which in turn participates in GCN5 HAT-containing complexes. Ada2a also produces an RNA polII subunit, RPB4, via alternative splicing; consequently, an overlapping regulatory region serves for the production of three proteins, each involved in transcription. By studying expression of reporter gene fusions in tissue culture cells and transgenic animals we have demonstrated that the regulatory regions of Ada2a/Rpb4 and Dtl overlap and the Dtl promoter is partly within the Ada2a/Rpb4 coding region. The shared regulatory region contains a DRE element, binding site of DREF, the protein factor involved in the regulation of a number of genes which play a role in DNA replication and cell proliferation. Despite the perfectly symmetrical DRE, DREF seems to have a more decisive role in Ada2a/Rpb4 transcription than in the transcription of Dtl.

KW - ADA2a

KW - DRE motif

KW - Drosophila promoter

KW - PIMT

UR - http://www.scopus.com/inward/record.url?scp=15744393418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15744393418&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2005.01.006

DO - 10.1016/j.gene.2005.01.006

M3 - Article

VL - 348

SP - 13

EP - 23

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2 SUPPL.

ER -