Intestinal smooth muscle is required for patterning the enteric nervous system

Hannah K. Graham, Ivy Maina, Allan M. Goldstein, N. Nagy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The development of the enteric nervous system (ENS) and intestinal smooth muscle occurs in a spatially and temporally correlated manner, but how they influence each other is unknown. In the developing mid-gut of the chick embryo, we find that α-smooth muscle actin expression, indicating early muscle differentiation, occurs after the arrival of migrating enteric neural crest-derived cells (ENCCs). In contrast, hindgut smooth muscle develops prior to ENCC arrival. Smooth muscle development is normal in experimentally aganglionic hindguts, suggesting that proper development and patterning of the muscle layers does not rely on the ENS. However, inhibiting early smooth muscle development severely disrupts ENS patterning without affecting ENCC proliferation or apoptosis. Our results demonstrate that early intestinal smooth muscle differentiation is required for patterning the developing ENS.

Original languageEnglish
Pages (from-to)567-574
Number of pages8
JournalJournal of Anatomy
Volume230
Issue number4
DOIs
Publication statusPublished - Apr 1 2017

Fingerprint

Enteric Nervous System
nervous system
smooth muscle
Smooth Muscle
muscle
neural crest
Muscle Development
Neural Crest
muscle development
hindgut
muscles
Chick Embryo
midgut
actin
Actins
cell proliferation
apoptosis
embryo (animal)
chicks
Cell Proliferation

Keywords

  • development
  • enteric nervous system
  • gut
  • neural crest
  • smooth muscle

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Intestinal smooth muscle is required for patterning the enteric nervous system. / Graham, Hannah K.; Maina, Ivy; Goldstein, Allan M.; Nagy, N.

In: Journal of Anatomy, Vol. 230, No. 4, 01.04.2017, p. 567-574.

Research output: Contribution to journalArticle

Graham, Hannah K. ; Maina, Ivy ; Goldstein, Allan M. ; Nagy, N. / Intestinal smooth muscle is required for patterning the enteric nervous system. In: Journal of Anatomy. 2017 ; Vol. 230, No. 4. pp. 567-574.
@article{ae89c7335ed14122bf4a05df7c246800,
title = "Intestinal smooth muscle is required for patterning the enteric nervous system",
abstract = "The development of the enteric nervous system (ENS) and intestinal smooth muscle occurs in a spatially and temporally correlated manner, but how they influence each other is unknown. In the developing mid-gut of the chick embryo, we find that α-smooth muscle actin expression, indicating early muscle differentiation, occurs after the arrival of migrating enteric neural crest-derived cells (ENCCs). In contrast, hindgut smooth muscle develops prior to ENCC arrival. Smooth muscle development is normal in experimentally aganglionic hindguts, suggesting that proper development and patterning of the muscle layers does not rely on the ENS. However, inhibiting early smooth muscle development severely disrupts ENS patterning without affecting ENCC proliferation or apoptosis. Our results demonstrate that early intestinal smooth muscle differentiation is required for patterning the developing ENS.",
keywords = "development, enteric nervous system, gut, neural crest, smooth muscle",
author = "Graham, {Hannah K.} and Ivy Maina and Goldstein, {Allan M.} and N. Nagy",
year = "2017",
month = "4",
day = "1",
doi = "10.1111/joa.12583",
language = "English",
volume = "230",
pages = "567--574",
journal = "Journal of Anatomy",
issn = "0021-8782",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Intestinal smooth muscle is required for patterning the enteric nervous system

AU - Graham, Hannah K.

AU - Maina, Ivy

AU - Goldstein, Allan M.

AU - Nagy, N.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - The development of the enteric nervous system (ENS) and intestinal smooth muscle occurs in a spatially and temporally correlated manner, but how they influence each other is unknown. In the developing mid-gut of the chick embryo, we find that α-smooth muscle actin expression, indicating early muscle differentiation, occurs after the arrival of migrating enteric neural crest-derived cells (ENCCs). In contrast, hindgut smooth muscle develops prior to ENCC arrival. Smooth muscle development is normal in experimentally aganglionic hindguts, suggesting that proper development and patterning of the muscle layers does not rely on the ENS. However, inhibiting early smooth muscle development severely disrupts ENS patterning without affecting ENCC proliferation or apoptosis. Our results demonstrate that early intestinal smooth muscle differentiation is required for patterning the developing ENS.

AB - The development of the enteric nervous system (ENS) and intestinal smooth muscle occurs in a spatially and temporally correlated manner, but how they influence each other is unknown. In the developing mid-gut of the chick embryo, we find that α-smooth muscle actin expression, indicating early muscle differentiation, occurs after the arrival of migrating enteric neural crest-derived cells (ENCCs). In contrast, hindgut smooth muscle develops prior to ENCC arrival. Smooth muscle development is normal in experimentally aganglionic hindguts, suggesting that proper development and patterning of the muscle layers does not rely on the ENS. However, inhibiting early smooth muscle development severely disrupts ENS patterning without affecting ENCC proliferation or apoptosis. Our results demonstrate that early intestinal smooth muscle differentiation is required for patterning the developing ENS.

KW - development

KW - enteric nervous system

KW - gut

KW - neural crest

KW - smooth muscle

UR - http://www.scopus.com/inward/record.url?scp=85014906131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014906131&partnerID=8YFLogxK

U2 - 10.1111/joa.12583

DO - 10.1111/joa.12583

M3 - Article

C2 - 28116763

AN - SCOPUS:85014906131

VL - 230

SP - 567

EP - 574

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

IS - 4

ER -