Intestinal ischemia-reperfusion leads to early systemic micro-rheological and multiorgan microcirculatory alterations in the rat

Anita Mester, Zsuzsanna Magyar, Viktoria Sogor, Bence Tanczos, Yoav Stark, Konstantin Cherniavsky, Laszlo Bidiga, Katalin Peto, N. Németh

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Intestinal ischemia-reperfusion (I/R) is a potentially life-threatening situation and its pathomechanism is not fully understood yet.

OBJECTIVE: To investigate the early micro-rheological, microcirculatory and morphological consequences of intestinal I/R in a rat model.

METHODS: CD rats were anesthetized and subjected to Control (n = 7) or I/R (n = 7) groups. Left femoral artery cannulation and median laparotomy were performed. In the I/R group the superior mesenteric artery was clamped for 30 minutes. Blood samples were taken before (Base) and after the ischemia, at the 30th, 60th and 120th minutes of the reperfusion (R-30, R-60, R-120). Hematological parameters, erythrocyte deformability and aggregation were determined. On the jejunum, the liver and the right kidney laser Doppler flowmetry tests were completed. At the end of experiment histological samples were taken.

RESULTS: Hematocrit, leukocyte and platelet counts increased during the reperfusion. Erythrocyte deformability worsened versus Control. All erythrocyte aggregation index values of I/R group increased gradually. Intestinal microcirculatory blood flux units (BFU) did not recover completely after ischemia, at R-30 liver BFU values were lower, and kidney values decreased by R-120. Histology showed signs of I/R injury.

CONCLUSIONS: Micro-rheological parameters may show early and significant deterioration during the reperfusion that might contribute further to microcirculatory alterations.

Original languageEnglish
Pages (from-to)35-44
Number of pages10
JournalClinical Hemorheology and Microcirculation
Volume68
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

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Reperfusion
Ischemia
Erythrocyte Aggregation
Erythrocyte Deformability
Kidney
Laser-Doppler Flowmetry
Erythrocyte Indices
Superior Mesenteric Artery
Liver
Jejunum
Femoral Artery
Reperfusion Injury
Platelet Count
Leukocyte Count
Hematocrit
Catheterization
Laparotomy
Histology

Keywords

  • hemorheology
  • Intestinal ischemia-reperfusion
  • microcirculation

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Intestinal ischemia-reperfusion leads to early systemic micro-rheological and multiorgan microcirculatory alterations in the rat. / Mester, Anita; Magyar, Zsuzsanna; Sogor, Viktoria; Tanczos, Bence; Stark, Yoav; Cherniavsky, Konstantin; Bidiga, Laszlo; Peto, Katalin; Németh, N.

In: Clinical Hemorheology and Microcirculation, Vol. 68, No. 1, 01.01.2018, p. 35-44.

Research output: Contribution to journalArticle

Mester, Anita ; Magyar, Zsuzsanna ; Sogor, Viktoria ; Tanczos, Bence ; Stark, Yoav ; Cherniavsky, Konstantin ; Bidiga, Laszlo ; Peto, Katalin ; Németh, N. / Intestinal ischemia-reperfusion leads to early systemic micro-rheological and multiorgan microcirculatory alterations in the rat. In: Clinical Hemorheology and Microcirculation. 2018 ; Vol. 68, No. 1. pp. 35-44.
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AU - Mester, Anita

AU - Magyar, Zsuzsanna

AU - Sogor, Viktoria

AU - Tanczos, Bence

AU - Stark, Yoav

AU - Cherniavsky, Konstantin

AU - Bidiga, Laszlo

AU - Peto, Katalin

AU - Németh, N.

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