Intestinal coelomic transplants

A novel method for studying enteric nervous system development

N. Nagy, Allan M. Goldstein

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Normal development of the enteric nervous system (ENS) requires the coordinated activity of multiple proteins to regulate the migration, proliferation, and differentiation of enteric neural crest cells. Much of our current knowledge of the molecular regulation of ENS development has been gained from transgenic mouse models and cultured neural crest cells. We have developed a method for studying the molecular basis of ENS formation complementing these techniques. Aneural quail or mouse hindgut, isolated prior to the arrival of neural crest cells, was transplanted into the coelomic cavity of a host chick embryo. Neural crest cells from the chick host migrated to and colonized the grafted hindgut. Thorough characterization of the resulting intestinal chimeras was performed by using immunohistochemistry and vital dye labeling to determine the origin of the host-derived cells, their pattern of migration, and their capacity to differentiate. The formation of the ENS in the intestinal chimeras was found to recapitulate many aspects of normal ENS development. The host-derived cells arose from the vagal neural crest and populated the graft in a rostral-to-caudal wave of migration, with the submucosal plexus being colonized first. These crest-derived cells differentiated into neurons and glial cells, forming ganglionated plexuses grossly indistinguishable from normal ENS. The resulting plexuses were specific to the grafted hindgut, with quail grafts developing two ganglionated plexuses, but mouse grafts developing only a single myenteric plexus. We discuss the advantages of intestinal coelomic transplants for studying ENS development.

Original languageEnglish
Pages (from-to)43-55
Number of pages13
JournalCell and Tissue Research
Volume326
Issue number1
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Enteric Nervous System
Transplants
Neurology
Neural Crest
Grafts
Quail
Submucous Plexus
Myenteric Plexus
Chick Embryo
Neuroglia
Labeling
Transgenic Mice
Neurons
Coloring Agents
Immunohistochemistry

Keywords

  • Coelomic graft
  • Enteric nervous system
  • Hindgut
  • Mouse-chick chimera
  • Quail-chick chimera

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Intestinal coelomic transplants : A novel method for studying enteric nervous system development. / Nagy, N.; Goldstein, Allan M.

In: Cell and Tissue Research, Vol. 326, No. 1, 10.2006, p. 43-55.

Research output: Contribution to journalArticle

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