Interspecies diversity of erythrocyte mechanical stability at various combinations in magnitude and duration of shear stress, and osmolality

N. Németh, Viktoria Sogor, Ferenc Kiss, Pinar Ulker

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We hypothesized that the results of red blood cell mechanical stability test show interspecies differences. The comparative investigations were performed on blood samples obtained from rats, beagle dogs, pigs and healthy volunteers. Mechanical stress was applied in nine combinations: 30, 60 or 100 Pa shear stress for 100, 200 or 300 seconds. Generally, rat erythrocytes showed the highest capability of resistance. With the applied combinations of mechanical stress pig erythrocytes were the most sensitive. On human erythrocytes 60 Pa for 200 s was the minimum combination to result significant deformability deterioration. By increasing the magnitude and duration of the applied mechanical stress we experienced escalating deformability impairment in all species. 100 Pa shear stress for 300 seconds on human erythrocytes showed the largest deformability impairment. The mechanical stability test results were also dependent on osmolality. At hypoosmolar range (200 mOsmol/kg) the mechanical stress improved EI data mostly in rat and porcine blood. At higher osmolality (500 mOsmol/kg), the test did not show detectable difference, while in 250-300 mOsmol/kg range the differences were well observable. In summary, erythrocytes' capability of resistance against mechanical stress shows interspecies differences depending on the magnitude and duration of the applied stress, and on the osmolality.

Original languageEnglish
Pages (from-to)381-398
Number of pages18
JournalClinical Hemorheology and Microcirculation
Volume63
Issue number4
DOIs
Publication statusPublished - 2016

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Mechanical Stress
Osmolar Concentration
Erythrocytes
Swine
Healthy Volunteers
Dogs

Keywords

  • comparative hemorheology
  • mechanical stability
  • membrane stability
  • osmotic gradient ektacytometry
  • Red blood cell deformability

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Interspecies diversity of erythrocyte mechanical stability at various combinations in magnitude and duration of shear stress, and osmolality. / Németh, N.; Sogor, Viktoria; Kiss, Ferenc; Ulker, Pinar.

In: Clinical Hemorheology and Microcirculation, Vol. 63, No. 4, 2016, p. 381-398.

Research output: Contribution to journalArticle

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