Interpretation of mtDNA RFLP variability among Aspergillus tubingensis isolates

Ákos Juhász, Helga Engi, I. Pfeiffer, J. Kucsera, C. Vágvölgyi, Z. Hamari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aspergillus tubingensis isolates collected from distant geographic areas were earlier classified into six groups on the basis of the mtDNA RFLP variability they exhibited (mtDNA types 2a-2f). In the present work, we investigated the reason for the intraspecific mtDNA variability and we describe here how this fungus, with a relatively small mitochondrial genome, can display intraspecific polymorphism due to intron acquisition and also sporadic point mutations affecting the recognition motifs of the restriction enzymes employed in the RFLP analysis. Three different LAGLI-DADG type group I introns were identified in the cox1 gene amongst the six mtDNA RFLP types. MtDNAs of types 2b and 2d contain all of the three introns, mtDNA of type 2f carries only one, and the other mtDNA types contain two introns each. Comparative analysis showed that the first and second introns of mtDNAs of types 2b and 2d are well distributed among fungi, indicating their active horizontal transfer capacity. The third intron occurs rarely among fungi and is restricted to a limited number of fungal species, namely to A. tubingensis and the yeast Candida stellata. It is interesting that this intron is present in a small mitochondrial genome such as that of A. tubingensis and, considering its rarity, its presence amongst black Aspergillus isolates is recommended to be considered as a tool to establish taxonomical unit(s) or to track down evolutionary divergence of closely related taxonomical units.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume91
Issue number3
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Aspergillus
Mitochondrial DNA
Restriction Fragment Length Polymorphisms
Introns
Fungi
Mitochondrial Genome
Candida
Point Mutation
Yeasts
Enzymes
Genes

Keywords

  • Aspergillus tubingensis
  • Cox 1 gene
  • Intron
  • mtDNA
  • Physical map
  • RFLP

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

@article{79bffbea511b46b0a48e3f3a5cceb589,
title = "Interpretation of mtDNA RFLP variability among Aspergillus tubingensis isolates",
abstract = "Aspergillus tubingensis isolates collected from distant geographic areas were earlier classified into six groups on the basis of the mtDNA RFLP variability they exhibited (mtDNA types 2a-2f). In the present work, we investigated the reason for the intraspecific mtDNA variability and we describe here how this fungus, with a relatively small mitochondrial genome, can display intraspecific polymorphism due to intron acquisition and also sporadic point mutations affecting the recognition motifs of the restriction enzymes employed in the RFLP analysis. Three different LAGLI-DADG type group I introns were identified in the cox1 gene amongst the six mtDNA RFLP types. MtDNAs of types 2b and 2d contain all of the three introns, mtDNA of type 2f carries only one, and the other mtDNA types contain two introns each. Comparative analysis showed that the first and second introns of mtDNAs of types 2b and 2d are well distributed among fungi, indicating their active horizontal transfer capacity. The third intron occurs rarely among fungi and is restricted to a limited number of fungal species, namely to A. tubingensis and the yeast Candida stellata. It is interesting that this intron is present in a small mitochondrial genome such as that of A. tubingensis and, considering its rarity, its presence amongst black Aspergillus isolates is recommended to be considered as a tool to establish taxonomical unit(s) or to track down evolutionary divergence of closely related taxonomical units.",
keywords = "Aspergillus tubingensis, Cox 1 gene, Intron, mtDNA, Physical map, RFLP",
author = "{\'A}kos Juh{\'a}sz and Helga Engi and I. Pfeiffer and J. Kucsera and C. V{\'a}gv{\"o}lgyi and Z. Hamari",
year = "2007",
month = "4",
doi = "10.1007/s10482-006-9110-x",
language = "English",
volume = "91",
pages = "209--216",
journal = "Antonie van Leeuwenhoek",
issn = "0003-6072",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Interpretation of mtDNA RFLP variability among Aspergillus tubingensis isolates

AU - Juhász, Ákos

AU - Engi, Helga

AU - Pfeiffer, I.

AU - Kucsera, J.

AU - Vágvölgyi, C.

AU - Hamari, Z.

PY - 2007/4

Y1 - 2007/4

N2 - Aspergillus tubingensis isolates collected from distant geographic areas were earlier classified into six groups on the basis of the mtDNA RFLP variability they exhibited (mtDNA types 2a-2f). In the present work, we investigated the reason for the intraspecific mtDNA variability and we describe here how this fungus, with a relatively small mitochondrial genome, can display intraspecific polymorphism due to intron acquisition and also sporadic point mutations affecting the recognition motifs of the restriction enzymes employed in the RFLP analysis. Three different LAGLI-DADG type group I introns were identified in the cox1 gene amongst the six mtDNA RFLP types. MtDNAs of types 2b and 2d contain all of the three introns, mtDNA of type 2f carries only one, and the other mtDNA types contain two introns each. Comparative analysis showed that the first and second introns of mtDNAs of types 2b and 2d are well distributed among fungi, indicating their active horizontal transfer capacity. The third intron occurs rarely among fungi and is restricted to a limited number of fungal species, namely to A. tubingensis and the yeast Candida stellata. It is interesting that this intron is present in a small mitochondrial genome such as that of A. tubingensis and, considering its rarity, its presence amongst black Aspergillus isolates is recommended to be considered as a tool to establish taxonomical unit(s) or to track down evolutionary divergence of closely related taxonomical units.

AB - Aspergillus tubingensis isolates collected from distant geographic areas were earlier classified into six groups on the basis of the mtDNA RFLP variability they exhibited (mtDNA types 2a-2f). In the present work, we investigated the reason for the intraspecific mtDNA variability and we describe here how this fungus, with a relatively small mitochondrial genome, can display intraspecific polymorphism due to intron acquisition and also sporadic point mutations affecting the recognition motifs of the restriction enzymes employed in the RFLP analysis. Three different LAGLI-DADG type group I introns were identified in the cox1 gene amongst the six mtDNA RFLP types. MtDNAs of types 2b and 2d contain all of the three introns, mtDNA of type 2f carries only one, and the other mtDNA types contain two introns each. Comparative analysis showed that the first and second introns of mtDNAs of types 2b and 2d are well distributed among fungi, indicating their active horizontal transfer capacity. The third intron occurs rarely among fungi and is restricted to a limited number of fungal species, namely to A. tubingensis and the yeast Candida stellata. It is interesting that this intron is present in a small mitochondrial genome such as that of A. tubingensis and, considering its rarity, its presence amongst black Aspergillus isolates is recommended to be considered as a tool to establish taxonomical unit(s) or to track down evolutionary divergence of closely related taxonomical units.

KW - Aspergillus tubingensis

KW - Cox 1 gene

KW - Intron

KW - mtDNA

KW - Physical map

KW - RFLP

UR - http://www.scopus.com/inward/record.url?scp=33947203750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947203750&partnerID=8YFLogxK

U2 - 10.1007/s10482-006-9110-x

DO - 10.1007/s10482-006-9110-x

M3 - Article

VL - 91

SP - 209

EP - 216

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

SN - 0003-6072

IS - 3

ER -