Interpretation of bafilomycin, pH neutralizing or protease inhibitor treatments in autophagic flux experiments: Novel considerations

Research output: Contribution to journalComment/debate

34 Citations (Scopus)

Abstract

Recent publications showed that the kinase MTOR localizes to lysosomes and its activation depends on amino acids inside the lysosomal lumen, implying that autophagic protein degradation is a positive regulator of MTOR in this setting. Since decreased MTOR activity results in autophagy induction, drug treatments that block autolysosomal degradation (a commonly used technique to estimate autophagic flux) may actually interfere not only with lysosomal breakdown, but also increase autophagosome generation through impaired MTOR signaling.

Original languageEnglish
Pages (from-to)1875-1876
Number of pages2
JournalAutophagy
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Atg8
  • Autophagy
  • Chloroquine
  • LC3
  • Leupeptin
  • Lysosome
  • MTOR
  • TOR
  • v-ATPase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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