Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi

Attila Pálvölgyi, Réka Sári, J. Németh, Annamária Szabolcs, István Nagy, P. Hegyi, J. Lonovics, Z. Szilvássy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). Methods: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric tension. The experiments were carried out in a thermostatically controlled (37±0.2 °C) organ bath (5 mL) containing Krebs solution. The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VIPa, 30 μmol/L), a VIP receptor antagonist. Results: CCK-8 stimulated the phasic activity of the SO. NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VIPa also attenuated this CCK-8 effect. The combined application of LNNA and VIPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. Conclusion: VIP and NO together contribute to an increase in phasic activity of SO.

Original languageEnglish
Pages (from-to)3264-3266
Number of pages3
JournalWorld Journal of Gastroenterology
Volume11
Issue number21
Publication statusPublished - Jun 7 2005

Fingerprint

Sphincter of Oddi
Sincalide
Vasoactive Intestinal Peptide
Nitric Oxide
Rabbits
Nitric Oxide Synthase
Muscles
Nitroarginine
Transducers
Baths
Duodenum
Bile
Fats

Keywords

  • CCK
  • LNNA
  • NO
  • Sphincter of Oddi
  • VIP

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi. / Pálvölgyi, Attila; Sári, Réka; Németh, J.; Szabolcs, Annamária; Nagy, István; Hegyi, P.; Lonovics, J.; Szilvássy, Z.

In: World Journal of Gastroenterology, Vol. 11, No. 21, 07.06.2005, p. 3264-3266.

Research output: Contribution to journalArticle

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abstract = "Aim: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). Methods: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric tension. The experiments were carried out in a thermostatically controlled (37±0.2 °C) organ bath (5 mL) containing Krebs solution. The organ fluid was gassed with 95{\%} O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VIPa, 30 μmol/L), a VIP receptor antagonist. Results: CCK-8 stimulated the phasic activity of the SO. NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VIPa also attenuated this CCK-8 effect. The combined application of LNNA and VIPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. Conclusion: VIP and NO together contribute to an increase in phasic activity of SO.",
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T1 - Interplay between nitric oxide and VIP in CCK-8-induced phasic contractile activity in the rabbit sphincter of Oddi

AU - Pálvölgyi, Attila

AU - Sári, Réka

AU - Németh, J.

AU - Szabolcs, Annamária

AU - Nagy, István

AU - Hegyi, P.

AU - Lonovics, J.

AU - Szilvássy, Z.

PY - 2005/6/7

Y1 - 2005/6/7

N2 - Aim: The sphincter of Oddi (SO) plays an important role in delivery of bile into the duodenum. To establish whether vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) were involved in phasic contractile activity of the rabbit SO stimulated by cholecystokinin-octapeptide (CCK-8). Methods: Isolated SO muscle rings were cleaned of fat and mounted horizontally on two small L-shaped hooks one of which was connected to a force transducer for the measurement of isometric tension. The experiments were carried out in a thermostatically controlled (37±0.2 °C) organ bath (5 mL) containing Krebs solution. The organ fluid was gassed with 95% O2 and 50 mL/L CO2 to keep the pH at 7.40±0.05. Contractile responses to CCK-8 (1 μmol/L) were evaluated in the presence and absence of NG-nitro-L-arginine (LNNA), an inhibitor of NO synthase (100 μmol/L), and (p-chloro-D-Phe6-Leu17)-VIP (VIPa, 30 μmol/L), a VIP receptor antagonist. Results: CCK-8 stimulated the phasic activity of the SO. NO synthase inhibition increased the frequency and amplitude of contractions with a slight increase in developed tension. Pre-incubation with VIPa also attenuated this CCK-8 effect. The combined application of LNNA and VIPa abolished the phasic activity of the muscle rings with a marked increase in tension in response to CCK-8. Conclusion: VIP and NO together contribute to an increase in phasic activity of SO.

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