Interneurons containing calretinin are specialized to control other interneurons in the rat hippocampus

Attila I. Gulyás, Norbert Hájos, T. Freund

Research output: Contribution to journalArticle

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Abstract

Spine-free calretinin-immunoreactive (CR-IR) interneurons form a subpopulation of GABAergic cells in the rat hippocampus. A characteristic feature of these cells-located in all areas and layers-is the frequent dendro-dendritic and axo-dendritic contacts they form with each other. In this study we examined in detail the connectivity of these neurons by reconstructing their dendritic and axonal arbor and by identifying their postsynaptic targets. Radially running dendrites of CR-IR cells, located in different layers, intermingled into long braids. An average cell was in contact with dendrites of three to seven other CR-IR cells. Reconstruction of the dendritic trees from six consecutive sections demonstrated that at least 15 cells may participate in a dendro-dendritically connected cluster. Electron microscopical examination revealed that regularly spaced zonula adherentia connect the touching dendrites. The postsynaptic targets of CR-IR neurons have been examined using postembedding immunogold staining for GABA. CR-containing GABA-immunoreactive axons of local origin formed multiple symmetrical synaptic contacts (two to five) exclusively on GABAergic dendrites (CR-negative as well as CR-positive). Two to 10 CR-IR axons may converge onto a single CR-IR neuron, often from cells belonging to the same dendro-dendritically connected cluster. Using double immunocytochemistry, CR- IR cells were shown to heavily innervate calbindin D(28k)-containing interneurons and VIP-containing basket cells but avoided the parvalbumin- containing basket and axo-axonic cells. The unique connectivity of CR-IR cells may enable them to play a crucial role in the generation of synchronous, rhythmic hippocampal activity by controlling other interneurons terminating on different dendritic and somatic compartments of principal cells.

Original languageEnglish
Pages (from-to)3397-3411
Number of pages15
JournalJournal of Neuroscience
Volume16
Issue number10
Publication statusPublished - May 15 1996

Fingerprint

Calbindin 2
Interneurons
Hippocampus
Dendrites
Neurons
gamma-Aminobutyric Acid
Axons
Calbindins
Parvalbumins
Running
Spine

Keywords

  • cholecystokinin
  • dendro-dendritic contacts
  • disinhibition
  • GABA
  • gap junction
  • inhibition
  • nonpyramidal cells
  • oscillation
  • VIP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interneurons containing calretinin are specialized to control other interneurons in the rat hippocampus. / Gulyás, Attila I.; Hájos, Norbert; Freund, T.

In: Journal of Neuroscience, Vol. 16, No. 10, 15.05.1996, p. 3397-3411.

Research output: Contribution to journalArticle

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