Internal quality control of an enzyme-linked immunoassay for Cry1Ab toxin detection applied in animal tissues

E. Takács, A. Nagy, Gelencsér, A. Székács

Research output: Contribution to journalArticle

Abstract

Reliable determination of microbial or transgenic Cry toxins is an essential issue in food and feed analyses, and enzyme-linked immunosorbent assays (ELISAs) are the method of choice for quantifying these toxins currently infood and environmental analysis. Internal Quality Control (IQC) is an indispensable method to assess accuracy, precision, and reproducibility of analytical measurements. To assess the utility of the ELISA method, IQC wasperformed on EnviroLogix Cry1Ab/Cry1Ac QualiPlate ELISA with manufacturer supplied analytical standards. Applicability of negative and positive controls (C- and C+) was examined by Shewhart Control Charts for bias andControl Chart of the Range of Duplicates for precision. Linear regression (up to 5 ng ml-1 Cry1Ab concentration) of the commercial ELISA kit was compared to sigmoid calibration (up to 60 ng ml-1 Cry1Ab concentration). Forimmunoassay optimization process, possible matrix effects in different liquid and solid vertebrate tissues were examined by determination of the limit of detection values in these matrices.

Original languageEnglish
Pages (from-to)593-600
Number of pages8
JournalActa Alimentaria
Volume44
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

animal tissues
immunoassays
Immunoenzyme Techniques
Quality Control
quality control
toxins
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
enzymes
Food Analysis
environmental assessment
Sigmoid Colon
reproducibility
Calibration
Vertebrates
Limit of Detection
Linear Models
detection limit
calibration
methodology

Keywords

  • Cry1Ab
  • ELISA
  • Internal quality control
  • Matrix effect
  • Porcine tissue

ASJC Scopus subject areas

  • Food Science

Cite this

Internal quality control of an enzyme-linked immunoassay for Cry1Ab toxin detection applied in animal tissues. / Takács, E.; Nagy, A.; Gelencsér, ; Székács, A.

In: Acta Alimentaria, Vol. 44, No. 4, 01.12.2015, p. 593-600.

Research output: Contribution to journalArticle

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