Internal motions in myosin head: Effect of ADP and ATP

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Internal flexibility of myosin heads in glycerinated muscle fibres in the presence of MgADP plus orthovanadate and after addition of Ca-ATP was studied using an isothiocyanate-based spin label attached to the reactive sulfhydryl sites of myosin. The spin labels were immobilized on the microsecond time scale and exhibited significant orientational order in rigor. In AM+.ADP.V(i) state a smaller fraction of ordered population was found showing distinct orientation from rigor; the larger population of heads was in dinamically disordered state. This new ordered population of heads was detected even in contracting fibres.

Original languageEnglish
Pages (from-to)936-940
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number3
DOIs
Publication statusPublished - Feb 27 1996

Fingerprint

Spin Labels
Myosins
Adenosine Diphosphate
Adenosine Triphosphate
Vanadates
Fibers
Head
Population
Muscle
Catalytic Domain
Muscles
isothiocyanic acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Internal motions in myosin head : Effect of ADP and ATP. / Belágyi, J.; Lőrinczy, D.

In: Biochemical and Biophysical Research Communications, Vol. 219, No. 3, 27.02.1996, p. 936-940.

Research output: Contribution to journalArticle

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