Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects

Ghislaine Gayan-Ramirez, Dries Testelmans, Karen Maes, Gábor Z. Rácz, Pascal Cadot, E. Zádor, Frank Wuytack, Marc Decramer

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Objective: Short-term mechanical ventilation has been proven to reduce diaphragm force and fiber dimensions. We hypothesized that intermittent spontaneous breathing during the course of mechanical ventilation would minimize the effects of mechanical ventilation on diaphragm force and expression levels of transcription factors (MyoD and myogenin). Design: Randomized, controlled experiment. Setting: Animal basic science laboratory. Subjects: Male Wistar rats, weighing 350-500 g. Interventions: Anesthetized and tracheotomized rats were submitted to either 24 hrs of spontaneous breathing (SB, n = 5), 24 hrs of continuous controlled mechanical ventilation (CMV, n = 7), or controlled mechanical ventilation with intermittent spontaneous breathing: 60 mins every 5 hrs of mechanical ventilation repeated four times (ISB60, n = 8) or 5 mins every 5 hrs 55 mins of mechanical ventilation repeated four times (SB5, n = 9). They were compared with control animals free from intervention (C, n = 5). Measurements and Main Results: The profile of the diaphragm force-frequency curve of the controls and SB group was significantly different from that of the ISB and CMV groups; especially, the mean asymptotic force was less in the ISB and CMV compared with controls and SB. CMV resulted in a significant decrease in the diaphragm type I (-26%, p <.05 vs. C) and type IIx/b (-39%, p <.005 vs. C and SB) cross-sectional area, whereas this was not observed in the ISB groups. Diaphragm MyoD protein expression was significantly decreased after ISB60 (-35%, p <.0001 vs. C and SB) and even more after CMV (-73%, p <.0001 vs. others). The same pattern was observed with myogenin protein levels. Positive relationships between diaphragm MyoD and myogenin protein levels and diaphragm force were observed. Conclusions: The data demonstrated that intermittent spontaneous breathing during the course of mechanical ventilation may minimize the deleterious effect of controlled mechanical ventilation on diaphragm force, fiber dimensions, and expression of transcription factors.

Original languageEnglish
Pages (from-to)2804-2809
Number of pages6
JournalCritical Care Medicine
Volume33
Issue number12
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Diaphragm
Artificial Respiration
Respiration
Myogenin
MyoD Protein
Transcription Factors
Laboratory Animal Science
Wistar Rats
Control Groups

Keywords

  • Diaphragm
  • Intermittent spontaneous breathing
  • Mechanical ventilation
  • Myogenic regulatory factors

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects. / Gayan-Ramirez, Ghislaine; Testelmans, Dries; Maes, Karen; Rácz, Gábor Z.; Cadot, Pascal; Zádor, E.; Wuytack, Frank; Decramer, Marc.

In: Critical Care Medicine, Vol. 33, No. 12, 12.2005, p. 2804-2809.

Research output: Contribution to journalArticle

Gayan-Ramirez, G, Testelmans, D, Maes, K, Rácz, GZ, Cadot, P, Zádor, E, Wuytack, F & Decramer, M 2005, 'Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects', Critical Care Medicine, vol. 33, no. 12, pp. 2804-2809. https://doi.org/10.1097/01.CCM.0000191250.32988.A3
Gayan-Ramirez, Ghislaine ; Testelmans, Dries ; Maes, Karen ; Rácz, Gábor Z. ; Cadot, Pascal ; Zádor, E. ; Wuytack, Frank ; Decramer, Marc. / Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects. In: Critical Care Medicine. 2005 ; Vol. 33, No. 12. pp. 2804-2809.
@article{badcb6addc5044b9a5466348b7ae187d,
title = "Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects",
abstract = "Objective: Short-term mechanical ventilation has been proven to reduce diaphragm force and fiber dimensions. We hypothesized that intermittent spontaneous breathing during the course of mechanical ventilation would minimize the effects of mechanical ventilation on diaphragm force and expression levels of transcription factors (MyoD and myogenin). Design: Randomized, controlled experiment. Setting: Animal basic science laboratory. Subjects: Male Wistar rats, weighing 350-500 g. Interventions: Anesthetized and tracheotomized rats were submitted to either 24 hrs of spontaneous breathing (SB, n = 5), 24 hrs of continuous controlled mechanical ventilation (CMV, n = 7), or controlled mechanical ventilation with intermittent spontaneous breathing: 60 mins every 5 hrs of mechanical ventilation repeated four times (ISB60, n = 8) or 5 mins every 5 hrs 55 mins of mechanical ventilation repeated four times (SB5, n = 9). They were compared with control animals free from intervention (C, n = 5). Measurements and Main Results: The profile of the diaphragm force-frequency curve of the controls and SB group was significantly different from that of the ISB and CMV groups; especially, the mean asymptotic force was less in the ISB and CMV compared with controls and SB. CMV resulted in a significant decrease in the diaphragm type I (-26{\%}, p <.05 vs. C) and type IIx/b (-39{\%}, p <.005 vs. C and SB) cross-sectional area, whereas this was not observed in the ISB groups. Diaphragm MyoD protein expression was significantly decreased after ISB60 (-35{\%}, p <.0001 vs. C and SB) and even more after CMV (-73{\%}, p <.0001 vs. others). The same pattern was observed with myogenin protein levels. Positive relationships between diaphragm MyoD and myogenin protein levels and diaphragm force were observed. Conclusions: The data demonstrated that intermittent spontaneous breathing during the course of mechanical ventilation may minimize the deleterious effect of controlled mechanical ventilation on diaphragm force, fiber dimensions, and expression of transcription factors.",
keywords = "Diaphragm, Intermittent spontaneous breathing, Mechanical ventilation, Myogenic regulatory factors",
author = "Ghislaine Gayan-Ramirez and Dries Testelmans and Karen Maes and R{\'a}cz, {G{\'a}bor Z.} and Pascal Cadot and E. Z{\'a}dor and Frank Wuytack and Marc Decramer",
year = "2005",
month = "12",
doi = "10.1097/01.CCM.0000191250.32988.A3",
language = "English",
volume = "33",
pages = "2804--2809",
journal = "Critical Care Medicine",
issn = "0090-3493",
publisher = "Lippincott Williams and Wilkins",
number = "12",

}

TY - JOUR

T1 - Intermittent spontaneous breathing protects the rat diaphragm from mechanical ventilation effects

AU - Gayan-Ramirez, Ghislaine

AU - Testelmans, Dries

AU - Maes, Karen

AU - Rácz, Gábor Z.

AU - Cadot, Pascal

AU - Zádor, E.

AU - Wuytack, Frank

AU - Decramer, Marc

PY - 2005/12

Y1 - 2005/12

N2 - Objective: Short-term mechanical ventilation has been proven to reduce diaphragm force and fiber dimensions. We hypothesized that intermittent spontaneous breathing during the course of mechanical ventilation would minimize the effects of mechanical ventilation on diaphragm force and expression levels of transcription factors (MyoD and myogenin). Design: Randomized, controlled experiment. Setting: Animal basic science laboratory. Subjects: Male Wistar rats, weighing 350-500 g. Interventions: Anesthetized and tracheotomized rats were submitted to either 24 hrs of spontaneous breathing (SB, n = 5), 24 hrs of continuous controlled mechanical ventilation (CMV, n = 7), or controlled mechanical ventilation with intermittent spontaneous breathing: 60 mins every 5 hrs of mechanical ventilation repeated four times (ISB60, n = 8) or 5 mins every 5 hrs 55 mins of mechanical ventilation repeated four times (SB5, n = 9). They were compared with control animals free from intervention (C, n = 5). Measurements and Main Results: The profile of the diaphragm force-frequency curve of the controls and SB group was significantly different from that of the ISB and CMV groups; especially, the mean asymptotic force was less in the ISB and CMV compared with controls and SB. CMV resulted in a significant decrease in the diaphragm type I (-26%, p <.05 vs. C) and type IIx/b (-39%, p <.005 vs. C and SB) cross-sectional area, whereas this was not observed in the ISB groups. Diaphragm MyoD protein expression was significantly decreased after ISB60 (-35%, p <.0001 vs. C and SB) and even more after CMV (-73%, p <.0001 vs. others). The same pattern was observed with myogenin protein levels. Positive relationships between diaphragm MyoD and myogenin protein levels and diaphragm force were observed. Conclusions: The data demonstrated that intermittent spontaneous breathing during the course of mechanical ventilation may minimize the deleterious effect of controlled mechanical ventilation on diaphragm force, fiber dimensions, and expression of transcription factors.

AB - Objective: Short-term mechanical ventilation has been proven to reduce diaphragm force and fiber dimensions. We hypothesized that intermittent spontaneous breathing during the course of mechanical ventilation would minimize the effects of mechanical ventilation on diaphragm force and expression levels of transcription factors (MyoD and myogenin). Design: Randomized, controlled experiment. Setting: Animal basic science laboratory. Subjects: Male Wistar rats, weighing 350-500 g. Interventions: Anesthetized and tracheotomized rats were submitted to either 24 hrs of spontaneous breathing (SB, n = 5), 24 hrs of continuous controlled mechanical ventilation (CMV, n = 7), or controlled mechanical ventilation with intermittent spontaneous breathing: 60 mins every 5 hrs of mechanical ventilation repeated four times (ISB60, n = 8) or 5 mins every 5 hrs 55 mins of mechanical ventilation repeated four times (SB5, n = 9). They were compared with control animals free from intervention (C, n = 5). Measurements and Main Results: The profile of the diaphragm force-frequency curve of the controls and SB group was significantly different from that of the ISB and CMV groups; especially, the mean asymptotic force was less in the ISB and CMV compared with controls and SB. CMV resulted in a significant decrease in the diaphragm type I (-26%, p <.05 vs. C) and type IIx/b (-39%, p <.005 vs. C and SB) cross-sectional area, whereas this was not observed in the ISB groups. Diaphragm MyoD protein expression was significantly decreased after ISB60 (-35%, p <.0001 vs. C and SB) and even more after CMV (-73%, p <.0001 vs. others). The same pattern was observed with myogenin protein levels. Positive relationships between diaphragm MyoD and myogenin protein levels and diaphragm force were observed. Conclusions: The data demonstrated that intermittent spontaneous breathing during the course of mechanical ventilation may minimize the deleterious effect of controlled mechanical ventilation on diaphragm force, fiber dimensions, and expression of transcription factors.

KW - Diaphragm

KW - Intermittent spontaneous breathing

KW - Mechanical ventilation

KW - Myogenic regulatory factors

UR - http://www.scopus.com/inward/record.url?scp=29144479229&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144479229&partnerID=8YFLogxK

U2 - 10.1097/01.CCM.0000191250.32988.A3

DO - 10.1097/01.CCM.0000191250.32988.A3

M3 - Article

C2 - 16352963

AN - SCOPUS:29144479229

VL - 33

SP - 2804

EP - 2809

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

IS - 12

ER -