Interleukin-8 and tumor necrosis factor-alpha are involved in human aortic endothelial cell migration: The possible role of these cytokines in human aortic aneurysmal blood vessel growth

Z. Szekanecz, M. R. Shah, L. A. Harlow, W. H. Pearce, A. E. Koch

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Angiogenesis, the growth and proliferation of blood vessels, may be important in the pathogenesis of atherosclerosis and thus in human atherosclerotic abdominal aortic aneurysms (AAAs). Endothelial migration or chemotaxis is a vital component of the angiogenic response. Here, human aortic endothelial cells (hAECs) were used to investigate the effect of AAA tissue supernatants on hAEC chemotaxis. AAA tissue conditioned media were found to be chemotactic for hAECs. We have previously shown that the angiogenic cytokines interleukin (IL)-8, and tumor necrosis factor (TNF)-α are present in AAAs and normal aortic explant conditioned media. Currently, we have found that basic fibroblast growth factor (bFGF) and platelet-derived growth factor can also be detected in these supernatants. In order to identify whether some of these soluble mediators contributed to the chemotactic activity of these supernatants, conditioned media were preincubated with either neutralizing anti-IL-8, anti-TNF-α, anti-bFGF antibodies or control serum. Anti-IL-8 and anti-TNF-α significantly inhibited AAA tissue supernatant-induced hAEC chemotaxis (p <0.05), while anti-bFGF did not (p not significant). These results indicate that IL-8 and TNF-α may be important in chemotactic activity for hAECs in vitro and possibly in AAA neovascularization. The abrogation of angiogenesis using neutralizing antibodies may be a future goal in the therapy of certain disease states such as AAA where angiogenesis plays an important role.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalPathobiology
Volume62
Issue number3
Publication statusPublished - 1994

Fingerprint

Endothelial cells
Abdominal Aortic Aneurysm
Blood vessels
Interleukin-8
Cell Movement
Blood Vessels
Endothelial Cells
Tumor Necrosis Factor-alpha
Cytokines
Fibroblast Growth Factor 2
Conditioned Culture Medium
Growth
Chemotaxis
Tissue
Platelet-Derived Growth Factor
Neutralizing Antibodies
Human Activities
Atherosclerosis
Antibodies
Serum

Keywords

  • Angiogenesis
  • Aortic aneurysm
  • Atherosclerosis
  • Chemotaxis
  • Cytokines
  • Endothelium

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Interleukin-8 and tumor necrosis factor-alpha are involved in human aortic endothelial cell migration : The possible role of these cytokines in human aortic aneurysmal blood vessel growth. / Szekanecz, Z.; Shah, M. R.; Harlow, L. A.; Pearce, W. H.; Koch, A. E.

In: Pathobiology, Vol. 62, No. 3, 1994, p. 134-139.

Research output: Contribution to journalArticle

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