Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons.

A. Szűcs, S. S. Rubakhin, G. B. Stefano, T. K. Hughes, K. S. Rózsa

Research output: Contribution to journalArticle

Abstract

The effect of interleukin-4 (IL-4) was studied on voltage-gated Ca-current of the neuron RPeD1 of Lymnaea stagnalis L. (Mollusca, Basommatophora), using two-microelectrode voltage-clamp method. It was found that: 1. The neuron RPeD1 possessed a high voltage-activated Ca-current characterized by -40 mV activation threshold and reaching its maximum at +5+5(-)+10 mV. The activation time constant of the current was found to be a monnexponential function of the membrane potential. Currents were almost completely inactivated within 200-300 ms. 2. IL-4 (10-200 U/ml) uniformly and reversibly increased the peak value of Ca-current in a dose- and time-dependent manner (at concentrations 75, 150 and 200 U/ml the potentiation was 5.3 +/- 1.8% (n = 3), 5.5 +/- 1. (n = 3) and 14.6 +/- 7.4% (n = 4), respectively. 3. No changes in the membrane resistance and holding current were observed during IL-4 application. 4. The time constants of activation and inactivation were not affected by IL-4. The maximal conductance as the only kinetic parameter was increased under the influence of IL-4. 5. The affect in modulation of HVA Ca-current caused by IL-4 was additive between the doses of 10-15o U/ml but saturated at concentrations > 200 U/ml. 6. IL-4 antiserum was a potent inhibitor for enhancement of Ca-current by IL-4.

Original languageEnglish
Pages (from-to)351-362
Number of pages12
JournalActa Biologica Hungarica
Volume46
Issue number2-4
Publication statusPublished - 1995

Fingerprint

Lymnaea
Interleukin-4
Neurons
Electric potential
Chemical activation
membrane
Membranes
Mollusca
Microelectrodes
Clamping devices
Kinetic parameters
inhibitor
Interleukin-10
Membrane Potentials
Immune Sera
Modulation
kinetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Szűcs, A., Rubakhin, S. S., Stefano, G. B., Hughes, T. K., & Rózsa, K. S. (1995). Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons. Acta Biologica Hungarica, 46(2-4), 351-362.

Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons. / Szűcs, A.; Rubakhin, S. S.; Stefano, G. B.; Hughes, T. K.; Rózsa, K. S.

In: Acta Biologica Hungarica, Vol. 46, No. 2-4, 1995, p. 351-362.

Research output: Contribution to journalArticle

Szűcs, A, Rubakhin, SS, Stefano, GB, Hughes, TK & Rózsa, KS 1995, 'Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons.', Acta Biologica Hungarica, vol. 46, no. 2-4, pp. 351-362.
Szűcs A, Rubakhin SS, Stefano GB, Hughes TK, Rózsa KS. Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons. Acta Biologica Hungarica. 1995;46(2-4):351-362.
Szűcs, A. ; Rubakhin, S. S. ; Stefano, G. B. ; Hughes, T. K. ; Rózsa, K. S. / Interleukin-4 potentiates voltage-activated Ca-currents in Lymnaea neurons. In: Acta Biologica Hungarica. 1995 ; Vol. 46, No. 2-4. pp. 351-362.
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