Interleukin-15 enhances HIV-1-driven polyclonal B-cell response in vitro

L. Kacani, G. M. Sprinzl, A. Erdei, M. P. Dierich

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26 Citations (Scopus)

Abstract

Interleukin-15 (IL-15) is a recently described cytokine, produced by monocytes/macrophages, with biological activities similar to IL-2. Since IL-15 was shown to stimulate human B-cell proliferation and immunoglobulin secretion, we investigated its effect on human B-cells stimulated with heat-inactivated human immunodeficiency virus type 1 (iHIV-1) in vitro. We observed a dose-dependent elevation of [3H]-thymidine incorporation and immunoglobulin production by B-cells incubated in the presence of iHIV-1. Moreover, IL-15 stimulated HIV-1-driven B-cell proliferation similarly to IL-2. As to immunoglobulin secretion, IL-15 was able to potentiate the stimulatory effect of IL-10. The highest amounts of iHIV caused a decrease in B-cell proliferation and immunoglobulin secretion to baseline levels, even in the presence of cytokines. These findings indicate that during the late stages of AIDS, when monocytes/ macrophages become the major site of viral production, IL-15, in concert with other monocyte-derived cytokines, may promote polyclonal B-cell activation and hypergammaglobulinaemia, which are frequently associated with HIV infection.

Original languageEnglish
Pages (from-to)162-172
Number of pages11
JournalExperimental and Clinical Immunogenetics
Volume16
Issue number3
DOIs
Publication statusPublished - Jun 1 1999

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Keywords

  • HIV-1
  • Hypergammaglobulinaemia
  • IgA
  • IgG
  • IgM
  • Interleukin-15

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Genetics(clinical)

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