Interleukin-1 receptors are differentially expressed in normal and psoriatic T cells

Attila Bebes, Ferenc Kovács-Sólyom, Judit Prihoda, Róbert Kui, L. Kemény, R. Gyulai

Research output: Contribution to journalArticle

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Abstract

This study was carried out to examine the possible role of interleukin-1 (IL-1) in the functional insufficiency of regulatory T cells in psoriasis, by comparing the expression of IL-1 receptors on healthy control and psoriatic T cells. Patients with moderate-to-severe chronic plaque psoriasis and healthy volunteers, matched in age and sex, were selected for all experiments. CD4 +CD25- effector and CD4+CD25+CD 127 low regulatory T cells were separated and used for the experiments. Expression of the mRNA of IL-1 receptors (IL-1R1, IL-1R2, and sIL-1R2) was determined by quantitative real-time RT-PCR. Cell surface IL-1 receptor expression was assessed by flow cytometry. Relative expression of the signal transmitting IL-1 receptor type 1 (IL-1R1) mRNA is higher in resting psoriatic effector and regulatory T cells, and activation induces higher IL-1R1 protein expression in psoriatic T cells than in healthy cells. Psoriatic regulatory and effector T cells express increased mRNA levels of the decoy IL-1 receptors (IL-1R2 and sIL-1R2) upon activation compared to healthy counterparts. Psoriatic T cells release slightly more sIL-1R2 into their surrounding than healthy T cells. In conclusion, changes in the expression of IL-1 receptors in psoriatic regulatory and effector T cells could contribute to the pathogenesis of psoriasis.

Original languageEnglish
Article number472625
JournalMediators of Inflammation
Volume2014
DOIs
Publication statusPublished - 2014

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Interleukin-1 Receptors
Regulatory T-Lymphocytes
T-Lymphocytes
Psoriasis
Messenger RNA
Interleukin-1 Type I Receptors
Interleukin-1
Real-Time Polymerase Chain Reaction
Healthy Volunteers
Flow Cytometry
Proteins

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Medicine(all)

Cite this

Interleukin-1 receptors are differentially expressed in normal and psoriatic T cells. / Bebes, Attila; Kovács-Sólyom, Ferenc; Prihoda, Judit; Kui, Róbert; Kemény, L.; Gyulai, R.

In: Mediators of Inflammation, Vol. 2014, 472625, 2014.

Research output: Contribution to journalArticle

Bebes, Attila ; Kovács-Sólyom, Ferenc ; Prihoda, Judit ; Kui, Róbert ; Kemény, L. ; Gyulai, R. / Interleukin-1 receptors are differentially expressed in normal and psoriatic T cells. In: Mediators of Inflammation. 2014 ; Vol. 2014.
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