Interleukin-1β stimulates growth hormone-releasing hormone receptor mRNA expression in the rat hypothalamus in vitro and in vivo

P. Taishi, A. De, J. Alt, J. Gardi, F. Obal, James M. Krueger

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Changes in growth hormone-releasing hormone (GHRH), GHRH-receptor (R), somatostatin and interleukin (IL)-1β mRNA levels were determined in fetal rat hypothalamic cultures after administration of IL-1β (1, 10, 100 ng/ml, 2h incubation), and in adult rat hypothalamus 5 h after intracerebroventricular injection of IL-1β (2.5 and 25 ng). IL-1β stimulated GHRH-R mRNA expression both in vitro (10 and 100 ng/ml) and in vivo (2.5 and 25 ng). Somatostatin mRNA was significantly stimulated and GHRH mRNA slightly reduced in vitro, while these mRNA species were not altered in vivo in response to IL-1β. IL-1β stimulated its own expression both in vitro (10 and 100 ng/ml) and in vivo (25 ng). IL-1β-induced mRNA responses occurred 2 h after treatment in vitro (incubation times, 30 min to 6 h). IL-1β also elicited slight GHRH releases in vitro. Up-regulation of hypothalamic GHRH-R by IL-1β may explain previous findings suggesting that IL-1β stimulates GHRH activity.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJournal of Neuroendocrinology
Volume16
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Interleukin-1
Hypothalamus
Growth Hormone-Releasing Hormone
Messenger RNA
Somatostatin
somatotropin releasing hormone receptor
In Vitro Techniques
Hypothalamic Hormones
Interleukin-17
Up-Regulation
Injections

Keywords

  • Cytokine
  • Sleep
  • Somatostatin
  • Somatotropic

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Interleukin-1β stimulates growth hormone-releasing hormone receptor mRNA expression in the rat hypothalamus in vitro and in vivo. / Taishi, P.; De, A.; Alt, J.; Gardi, J.; Obal, F.; Krueger, James M.

In: Journal of Neuroendocrinology, Vol. 16, No. 2, 02.2004, p. 113-118.

Research output: Contribution to journalArticle

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