Interlaboratory comparison trial on cylindrospermopsin measurement

Andrea Törökné, Maria Asztalos, Maria Bánkiné, Heike Bickel, Georg Borbély, Shmuel Carmeli, Geoffrey A. Codd, Jutta Fastner, Qiong Huang, Andrew Humpage, James S. Metcalf, Erzsebet Rábai, Assaf Sukenik, Gyula Surányi, Gabor Vasas, Viktor Weiszfeiler

Research output: Contribution to journalArticle

43 Citations (Scopus)


The hepatotoxin cylindrospermopsin (CYN) is a potent inhibitor of protein synthesis in mammalian cells. It is produced by freshwater cyanobacterial blooms in countries such as Australia, the United States, Israel, Thailand, and Brazil. An interlaboratory comparison was organized as a first step to evaluate the measurement of CYN in lyophilized cyanobacterial cells. Six laboratories from Europe, Israel, and Australia participated in the trial. All of the methods used for extraction of the toxin and the high-performance liquid chromatography (HPLC) analysis were satisfactory on the basis of statistical evaluation, according to ISO standards 5725-1 and -2. Further comparison of all the extraction methods by the organizer indicated that the most effective extraction procedure used 5% formic acid to prevent interference in chromatograms by contaminant compounds when analyzed using HPLC employing isocratic conditions of 5% (v/v) aqueous methanol plus 0.1% (v/v) trifluoroacetic acid as the mobile phase.

Original languageEnglish
Pages (from-to)280-284
Number of pages5
JournalAnalytical Biochemistry
Issue number2
Publication statusPublished - Sep 15 2004


  • Cyanobacteria
  • Cylindrospermopsin
  • HPLC analysis
  • Intercomparison

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Törökné, A., Asztalos, M., Bánkiné, M., Bickel, H., Borbély, G., Carmeli, S., Codd, G. A., Fastner, J., Huang, Q., Humpage, A., Metcalf, J. S., Rábai, E., Sukenik, A., Surányi, G., Vasas, G., & Weiszfeiler, V. (2004). Interlaboratory comparison trial on cylindrospermopsin measurement. Analytical Biochemistry, 332(2), 280-284.