Intergeneric gene transfer mediated by plant protoplast fusion

D. Dudits, Olga Fejér, Gyula Hadlaczky, Csaba Koncz, Gábor B. Lázár, G. Horváth

Research output: Contribution to journalArticle

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Abstract

In attempts at somatic transfer of plant genomes of reduced size, X-irradiated leaf protoplasts of parsley (Petroselinum hortense, 2 n=22) were fused with cell culture protoplasts of a nuclear albino mutant of carrot (Daucus carota, 2 n=18). Introduction of genes from the irradiated parsley nuclei into the carrot genome was shown by the correction of the albino defect and by the appearance of parsley isoenzymes in selected green tissues and plants. The cytological studies provided information on significant deviation from the amphidiploid chromosome number. The high frequency of cells with 2 n=19, 2 n=38 and regeneration of plants with 2 n=19 chromosomes can indicate that the elimination of parsley chromosomes is incomplete. A correlation was found between the lethality of selected tissues and differentiated or undifferentiated stages of the cells.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalMGG Molecular & General Genetics
Volume179
Issue number2
DOIs
Publication statusPublished - Sep 1980

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Petroselinum
Protoplasts
Daucus carota
Genes
Chromosomes
Viridiplantae
Plant Genome
Chromosomes, Human, Pair 19
Genome Size
Isoenzymes
Regeneration
Cell Culture Techniques
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Intergeneric gene transfer mediated by plant protoplast fusion. / Dudits, D.; Fejér, Olga; Hadlaczky, Gyula; Koncz, Csaba; Lázár, Gábor B.; Horváth, G.

In: MGG Molecular & General Genetics, Vol. 179, No. 2, 09.1980, p. 283-288.

Research output: Contribution to journalArticle

Dudits, D. ; Fejér, Olga ; Hadlaczky, Gyula ; Koncz, Csaba ; Lázár, Gábor B. ; Horváth, G. / Intergeneric gene transfer mediated by plant protoplast fusion. In: MGG Molecular & General Genetics. 1980 ; Vol. 179, No. 2. pp. 283-288.
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