The Sendai virus induced interferon (IF) production by partially purified human leukocyte suspensions of normal donors and of leukemic patients was investigated in vitro. An increased production was observed with leukocytes taken from patients suffering from chronic myelogenous leukemia (CML) during exacerbation, but the production was approximately normal with cells taken during remission. IF production was not influenced by large doses of cytostatics (DBM, 5 FU, FUDR, 5 HU, 6 MP, (cyclophosphamide) irrespective of whether normal or CML leukocytes were used. In contrast, production was inhibited in both systems by inhibitors of RNA or protein synthesis (actinomycin D, puromycin, cycloheximide). CML leukocytes produced IF for a prolonged period of time as compared to normal leukocytes. Leukocytes from children with acute blastic leukemia and those from adults with chronic lymphoid or acute paramyeloblastic leukemia produce, in contrast to normal leukocytes, approximately as much IF in the absence as in the presence of serum. It is concluded that the Sendai virus induced IF synthesis in CML leukocytes requires de novo protein synthesis.
|Number of pages||8|
|Journal||Acta microbiologica Academiae Scientiarum Hungaricae|
|Publication status||Published - Dec 1 1976|
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