Interferon-induced modulation of human ras oncogene expression.

D. Samid, Z. Schaff, E. H. Chang, R. M. Friedman

Research output: Contribution to journalArticle

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Abstract

Treatment of NIH 3T3 cells with interferon (IFN) after transfection with human bladder carcinoma EJ/T24 c-Ha-ras1 oncogene DNA caused inhibition of ras-induced cell transformation. Furthermore, the effect of IFN on oncogene expression in an established tumor line was studied. A tumor line of NIH 3T3 (RS485) was transformed by human c-Ha-ras1 activated by a viral long terminal repeat. Treatment of the tumor cells with IFN was associated with a progressive appearance of reverted, flat colonies which exhibited a normal phenotype with respect to morphology and growth. The revertants were well spread, contact inhibited cells; they did not grow in soft agar and were not tumorigenic in nude mice. Revertants retained their normal phenotype, although they contained transfecting human c-Ha-ras1 DNA; but, they produced significantly decreased levels of the onc-encoded protein p21 and c-Ha-ras1 mRNA as compared to RS485 cells.

Original languageEnglish
Pages (from-to)265-268
Number of pages4
JournalProgress in Clinical and Biological Research
Volume192
Publication statusPublished - 1985

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ras Genes
Interferons
Oncogenes
Phenotype
Neoplasms
NIH 3T3 Cells
Terminal Repeat Sequences
DNA
Nude Mice
Agar
Transfection
Urinary Bladder
Carcinoma
Messenger RNA
Growth
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Interferon-induced modulation of human ras oncogene expression. / Samid, D.; Schaff, Z.; Chang, E. H.; Friedman, R. M.

In: Progress in Clinical and Biological Research, Vol. 192, 1985, p. 265-268.

Research output: Contribution to journalArticle

Samid, D. ; Schaff, Z. ; Chang, E. H. ; Friedman, R. M. / Interferon-induced modulation of human ras oncogene expression. In: Progress in Clinical and Biological Research. 1985 ; Vol. 192. pp. 265-268.
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