Objectives: This study was performed to further define the effects of interferon-γ on choriocarcinoma cell lines and to determine whether variations in response among cell lines are attributable to quantitative differences in interferon-γ receptors. Study design: Interferon-γ receptors were quantified on BeWo, JEG-3 and Jar choriocarcinoma cell lines by a radiolabeled interferon-γ ligand binding assay. The response of these cell lines to interferon-γ was measured in two functional assays: a cell proliferation assay and a cell lysis assay after exposure to interferon-γ with and without actinomycin-D. Results: The number of interferon-γ receptors on BeWo, Jar, and JEG-3 cells did not differ significantly (650, 560, and 420 interferon-γ receptors per cell, respectively). Proliferation of all three choriocarcinoma cell lines was significantly inhibited to a similar extent by interferon-γ. After treatment with interferon-γ actinomycin-D, each choriocarcinoma cell line exhibited dose-dependent cell lysis; lysis of Jar was significantly less than that of either BeWo or JEG-3. Conclusion: These data further document variations in the response of choriocarcinoma cell lines to interferon-γ and indicate that these differences are not the result of interferon-γ receptor number but of postreceptor mechanisms.
ASJC Scopus subject areas
- Obstetrics and Gynaecology