Interferon γ induces synthesis of complement alternative pathway proteins by human endothelial cells in culture

J. Ripoche, J. A. Mitchell, A. Erdei, C. Madin, B. Moffatt, T. Mokoena, S. Gordon, R. B. Sim

Research output: Contribution to journalArticle

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Abstract

Human umbilical vein endothelial cells grown in vitro under standard conditions contain a high level of mRNA specific for the complement regulatory factors H and I. An additional 1.8-kb mRNA encoding a truncated form of factor H is also present. IFN-γ stimulation of the cells causes a 6-7-fold increase in both factor H mRNA species, and a >10-fold increase in factor I mRNA. IL-1 and LPS slightly suppressed factor H mRNA, while TNF had no effect. mRNA for factor B is also detectable in IFN-γ-stimulated cells, but messengers for C1q, C4bp, and CR3β chain were not found. Secretion of factor H protein was also stimulated by IFN-γ. The presence of mRNA for factors H, B, and I, together with C3 secretion, demonstrated by others, suggests that endothelial cells can assemble the complete alternative complement pathway. Endothelial cell complement may be involved in leukocyte-endothelium interactions mediated by leukocyte C3 receptors.

Original languageEnglish
Pages (from-to)1917-1922
Number of pages6
JournalJournal of Experimental Medicine
Volume168
Issue number5
Publication statusPublished - 1988

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Alternative Complement Pathway
Complement Factor H
Interferons
Endothelial Cells
Cell Culture Techniques
Messenger RNA
Proteins
Complement Factor I
Leukocytes
Complement Factor B
Human Umbilical Vein Endothelial Cells
Interleukin-1
Endothelium

ASJC Scopus subject areas

  • Immunology

Cite this

Ripoche, J., Mitchell, J. A., Erdei, A., Madin, C., Moffatt, B., Mokoena, T., ... Sim, R. B. (1988). Interferon γ induces synthesis of complement alternative pathway proteins by human endothelial cells in culture. Journal of Experimental Medicine, 168(5), 1917-1922.

Interferon γ induces synthesis of complement alternative pathway proteins by human endothelial cells in culture. / Ripoche, J.; Mitchell, J. A.; Erdei, A.; Madin, C.; Moffatt, B.; Mokoena, T.; Gordon, S.; Sim, R. B.

In: Journal of Experimental Medicine, Vol. 168, No. 5, 1988, p. 1917-1922.

Research output: Contribution to journalArticle

Ripoche, J, Mitchell, JA, Erdei, A, Madin, C, Moffatt, B, Mokoena, T, Gordon, S & Sim, RB 1988, 'Interferon γ induces synthesis of complement alternative pathway proteins by human endothelial cells in culture', Journal of Experimental Medicine, vol. 168, no. 5, pp. 1917-1922.
Ripoche, J. ; Mitchell, J. A. ; Erdei, A. ; Madin, C. ; Moffatt, B. ; Mokoena, T. ; Gordon, S. ; Sim, R. B. / Interferon γ induces synthesis of complement alternative pathway proteins by human endothelial cells in culture. In: Journal of Experimental Medicine. 1988 ; Vol. 168, No. 5. pp. 1917-1922.
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