Interfacial shear rheological behaviour of proteins in three-phase partitioning systems

Réka Borbás, Brent S. Murray, E. Kiss

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Three-phase partitioning process (TPP) is a relatively recent, but fast developing technique, which is used for the accumulation of proteins from multicomponent extracts. In this process a coherent, protein-rich middle layer is formed between two immiscible liquid phases. The two liquid phases are developed by phase separation of systems consisting of tert-butanol, ammonium sulphate and water, and the system is characterised by low interfacial tension. Interfacial rheological properties of four different proteins (bovine serum albumin, ovalbumin, β-lactoglobulin and lysozyme) were investigated in model systems of TPP by means of a two-dimensional Couette-type interfacial rheometer used in the oscillatory mode. Ovalbumin and β-lactoglobulin showed the highest interfacial shear elasticity and viscosity, whilst the rheological parameters of lysozyme were hardly measurable. Shear viscoelastic behaviour of protein film adsorbed at the interface and of thin emulsion layer obtained by slight mixing of the liquid phases were compared as a function of time. The interfacial rheological results and also the microscopic observation of the middle layer support the crucial role of emulsification and emulsion stability related to viscoelastic character of the interfacial film in the protein separation during TPP.

Original languageEnglish
Pages (from-to)93-103
Number of pages11
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume213
Issue number1
DOIs
Publication statusPublished - Feb 4 2003

Fingerprint

shear
proteins
Proteins
Lactoglobulins
liquid phases
lysozyme
Ovalbumin
Muramidase
Emulsions
emulsions
Liquids
Enzymes
tert-Butyl Alcohol
ammonium sulfates
rheometers
Emulsification
Rheometers
Ammonium Sulfate
Bovine Serum Albumin
Butenes

Keywords

  • Emulsion gel
  • Interfacial rheology
  • Protein adsorption
  • Three-phase partitioning

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Interfacial shear rheological behaviour of proteins in three-phase partitioning systems. / Borbás, Réka; Murray, Brent S.; Kiss, E.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 213, No. 1, 04.02.2003, p. 93-103.

Research output: Contribution to journalArticle

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