Interfacial interactions between poly[L-lysine]-based branched polypeptides and phospholipid model membranes

R. Szabó, F. Hudecz, F. Reig

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The interaction of five poly[L-lysine]-derived branched chain polypeptides of poly[Lys(Xi)] (XiK) or poly[Lys(X i-DL-Alam)] (XAK) with lipid bilayers (DPPC and DPPC/PG, 8:2) was studied by fluorescence polarization techniques. Two fluorescent probes, DPH and TMA-DPH, were utilized to monitor changes of motion in the internal and/or in the polar head regions, respectively. Results indicate that the interaction of polypeptides with neutral (DPPC) bilayers is mainly dependent on the polarity and electrical charge of side chains. The amphoteric EiK shows the highest level of interaction. Polycationic polypeptides (HiK, PiK, TAK) have a relatively small effect on the transition temperature of the lipids, while the polyanionic Succ-EAK has no effect at the alkyl chain region of the bilayer. Data with TMA-DPH indicate the lack of pronounced interaction between the polypeptides and the outer surface of the liposome. Similar tendency was documented for DPPC/PG vesicles. Polypeptides, HiK, and PiK induce significant changes in the transition temperature, thus indicating their insertion into the hydrophobic core of the bilayer without marked effect on the polar head region. Results suggest that these polypeptides (except E iK) have no destabilizing effect on liposomes studied. These properties are considered as beneficial for their use as safe carriers for bioactive molecules.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalJournal of Colloid and Interface Science
Volume267
Issue number1
DOIs
Publication statusPublished - Nov 1 2003

Fingerprint

lysine
Polypeptides
Phospholipids
polypeptides
Lysine
membranes
Membranes
Peptides
Liposomes
interactions
Superconducting transition temperature
lipids
transition temperature
Lipid bilayers
activity (biology)
Fluorescent Dyes
Lipids
insertion
polarity
tendencies

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Interfacial interactions between poly[L-lysine]-based branched polypeptides and phospholipid model membranes. / Szabó, R.; Hudecz, F.; Reig, F.

In: Journal of Colloid and Interface Science, Vol. 267, No. 1, 01.11.2003, p. 18-24.

Research output: Contribution to journalArticle

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