Interfacial enzyme partitioning as a tool for constructing biosensors

N. Adányi, J. Szamos, E. E. Szabó, M. Váradi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To explore new possibilities of enzyme immobilization, we investigated bioactive layers prepared by a new procedure based on three-phase partitioning (TPP) of proteins. By this method a third phase or midlayer as a protein layer can be developed at the interface of a protein system containing two phases (organic solvent/aqueous salt solution). Proteins of meat origin partitioned together with bioselective material (e.g. an enzyme) after centrifugation resulted in excellent bioactive layers. In the newly developed sensor, glucose oxidase was immobilized in a layer, which was fixed on the surface of a platinum ring electrode. The biosensor was built in a flow injection analyzer (FIA) system, where the hydrogen peroxide generated during the enzymatic reactions was determined by an amperometric cell. The parameters for biochemical and electrochemical reactions (ion concentration and pH of buffer, flow rate) were optimized. The linear range of analysis by the newly developed sensor was from 0.5 to 10 mmol 1-1 glucose. The biosensor could be used for more than 300 analysis.

Original languageEnglish
Pages (from-to)329-338
Number of pages10
JournalActa Alimentaria
Volume28
Issue number4
Publication statusPublished - Dec 1999

Fingerprint

biosensors
Biosensing Techniques
sensors (equipment)
Enzymes
enzymes
meat protein
glucose oxidase
immobilized enzymes
platinum
Proteins
proteins
enzymatic reactions
electrodes
Glucose Oxidase
centrifugation
hydrogen peroxide
buffers
Platinum
Centrifugation
Immobilization

Keywords

  • Amperometric cell
  • Bioactive protein gel layer
  • FIA system
  • Three phase partitioning

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Interfacial enzyme partitioning as a tool for constructing biosensors. / Adányi, N.; Szamos, J.; Szabó, E. E.; Váradi, M.

In: Acta Alimentaria, Vol. 28, No. 4, 12.1999, p. 329-338.

Research output: Contribution to journalArticle

Adányi, N. ; Szamos, J. ; Szabó, E. E. ; Váradi, M. / Interfacial enzyme partitioning as a tool for constructing biosensors. In: Acta Alimentaria. 1999 ; Vol. 28, No. 4. pp. 329-338.
@article{ffc1716ec89e4899866cf83d6ce249ce,
title = "Interfacial enzyme partitioning as a tool for constructing biosensors",
abstract = "To explore new possibilities of enzyme immobilization, we investigated bioactive layers prepared by a new procedure based on three-phase partitioning (TPP) of proteins. By this method a third phase or midlayer as a protein layer can be developed at the interface of a protein system containing two phases (organic solvent/aqueous salt solution). Proteins of meat origin partitioned together with bioselective material (e.g. an enzyme) after centrifugation resulted in excellent bioactive layers. In the newly developed sensor, glucose oxidase was immobilized in a layer, which was fixed on the surface of a platinum ring electrode. The biosensor was built in a flow injection analyzer (FIA) system, where the hydrogen peroxide generated during the enzymatic reactions was determined by an amperometric cell. The parameters for biochemical and electrochemical reactions (ion concentration and pH of buffer, flow rate) were optimized. The linear range of analysis by the newly developed sensor was from 0.5 to 10 mmol 1-1 glucose. The biosensor could be used for more than 300 analysis.",
keywords = "Amperometric cell, Bioactive protein gel layer, FIA system, Three phase partitioning",
author = "N. Ad{\'a}nyi and J. Szamos and Szab{\'o}, {E. E.} and M. V{\'a}radi",
year = "1999",
month = "12",
language = "English",
volume = "28",
pages = "329--338",
journal = "Acta Alimentaria",
issn = "0139-3006",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - Interfacial enzyme partitioning as a tool for constructing biosensors

AU - Adányi, N.

AU - Szamos, J.

AU - Szabó, E. E.

AU - Váradi, M.

PY - 1999/12

Y1 - 1999/12

N2 - To explore new possibilities of enzyme immobilization, we investigated bioactive layers prepared by a new procedure based on three-phase partitioning (TPP) of proteins. By this method a third phase or midlayer as a protein layer can be developed at the interface of a protein system containing two phases (organic solvent/aqueous salt solution). Proteins of meat origin partitioned together with bioselective material (e.g. an enzyme) after centrifugation resulted in excellent bioactive layers. In the newly developed sensor, glucose oxidase was immobilized in a layer, which was fixed on the surface of a platinum ring electrode. The biosensor was built in a flow injection analyzer (FIA) system, where the hydrogen peroxide generated during the enzymatic reactions was determined by an amperometric cell. The parameters for biochemical and electrochemical reactions (ion concentration and pH of buffer, flow rate) were optimized. The linear range of analysis by the newly developed sensor was from 0.5 to 10 mmol 1-1 glucose. The biosensor could be used for more than 300 analysis.

AB - To explore new possibilities of enzyme immobilization, we investigated bioactive layers prepared by a new procedure based on three-phase partitioning (TPP) of proteins. By this method a third phase or midlayer as a protein layer can be developed at the interface of a protein system containing two phases (organic solvent/aqueous salt solution). Proteins of meat origin partitioned together with bioselective material (e.g. an enzyme) after centrifugation resulted in excellent bioactive layers. In the newly developed sensor, glucose oxidase was immobilized in a layer, which was fixed on the surface of a platinum ring electrode. The biosensor was built in a flow injection analyzer (FIA) system, where the hydrogen peroxide generated during the enzymatic reactions was determined by an amperometric cell. The parameters for biochemical and electrochemical reactions (ion concentration and pH of buffer, flow rate) were optimized. The linear range of analysis by the newly developed sensor was from 0.5 to 10 mmol 1-1 glucose. The biosensor could be used for more than 300 analysis.

KW - Amperometric cell

KW - Bioactive protein gel layer

KW - FIA system

KW - Three phase partitioning

UR - http://www.scopus.com/inward/record.url?scp=0033472723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033472723&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 329

EP - 338

JO - Acta Alimentaria

JF - Acta Alimentaria

SN - 0139-3006

IS - 4

ER -