Interactions of pathological hallmark proteins: Tubulin polymerization promoting protein/p25,β-amyloid, and α-synuclein

Judit Oláh, Orsolya Vincze, Dezso Virók, Dóra Simon, Zsolt Bozsó, Natália Tokési, István Horváth, Emma Hlavanda, János Kovács, Anna Magyar, Mária Szucs, Ferenc Orosz, Botond Penke, Judit Ovádi

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The disordered tubulin polymerization promoting protein (TPPP/p25) was found to be co-enriched in neuronal and glial inclusions with α-synuclein in Parkinson disease and multiple system atrophy, respectively; however, co-occurrence of α-synuclein with β-amyloid (Aβ) in human brain inclusions has been recently reported, suggesting the existence of mixed type pathologies that could result in obstacles in the correct diagnosis and treatment. Here we identified TPPP/p25 as an interacting partner of the soluble Aβ oligomers as major risk factors for Alzheimer disease using ProtoArray human protein microarray. The interactions of oligomeric Aβ with proteins involved in the etiology of neurological disorders were characterized by ELISA, surface plasmon resonance, pelleting experiments, and tubulin polymerization assay. We showed that the Aβ 42 tightly bound to TPPP/p25 (K d = 85 nM) and caused aberrant protein aggregation by inhibiting the physiologically relevant TPPP/p25-derived microtubule assembly. The pair-wise interactions of Aβ 42, α-synuclein, and tubulin were found to be relatively weak; however, these three components formed soluble ternary complex exclusively in the absence of TPPP/p25. The aggregation-facilitating activity of TPPP/p25 and its interaction with Aβ was monitored by electron microscopy with purified proteins by pelleting experiments with cell-free extracts as well as by confocal microscopy with CHO cells expressing TPPP/p25 or amyloid. The finding that the interaction of TPPP/p25 with Aβ can produce pathological-like aggregates is tightly coupled with unusual pathology of the Alzheimer disease revealed previously; that is, partial co-localization of Aβ and TPPP/p25 in the case of diffuse Lewy body disease with Alzheimer disease.

Original languageEnglish
Pages (from-to)34088-34100
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number39
DOIs
Publication statusPublished - Oct 30 2011

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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