Interactions of insulin-mimetic vanadium complexes with the cell constituents ATP and glutathione

Ágnes Dörnyei, Susana Marcão, João Costa Pessoa, Tamás Jakusch, Tamás Kiss

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32 Citations (Scopus)

Abstract

In order to assess the molecular form of vanadium insulin-mimetic complexes in cells, the interactions of VIVO-maltolate and V IVO-dipicolinate systems with adenosine 5′-triphosphate (ATP) and glutathione (GSH) in aqueous solution were studied by employing pH potentiometry, and EPR, CD and UV/Vis spectroscopy. The stoichiometries and stability constants of the complexes formed were determined at 25°C with an ionic strength of I = 0.2 mol dm-3 (KCl). The most probable binding mode of the complexes formed in solution was determined by means of various spectral methods. The results suggest that from among the important cell constituents, GSH mostly takes part in the reduction of VV to V IV and helps keep VIV in this oxidation state. ATP, which is a strong VIVO binder, chelates the metal ion, forming binary and/or ternary complexes. The results of this work strongly suggest that ATP binds relevant VIVO species under cellular conditions and thus might somehow be involved in the insulin-mimetic action of VIVO compounds.

Original languageEnglish
Pages (from-to)3614-3621
Number of pages8
JournalEuropean Journal of Inorganic Chemistry
Issue number18
DOIs
Publication statusPublished - Sep 18 2006

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Keywords

  • Coordination modes
  • Insulin mimetics
  • Vanadium oxido complexes

ASJC Scopus subject areas

  • Inorganic Chemistry

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