Interactions between tombusviruses and satellite RNAs of tomato bushy stunt virus: A defect in sat RNA B1 replication maps to ORF1 of a helper virus

Ana Célix, J. Burgyán, Emilio Rodríguez-Cerezo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The biological properties of two recently described satellite RNAs of tomato bushy stunt virus (TBSV) were analyzed in natural and experimental hosts. Full-length cDNA clones were constructed for sat RNAs B1 (822 nt) and B10 (612 nt) and used in inoculations with satellite-free transcripts of different tombusviruses. In all virus-host combinations tested, TBSV sat RNA B10 drastically reduced the accumulation of viral genomic RNA and attenuated symptoms. In contrast, sat RNA B1 caused a less marked reduction of viral RNA level and did not have any effect on symptoms. Experiments with Nicotiana benthamiana protoplasts showed that the differential effects of sat RNAs B1 and B10 on TBSV titer were related to differential abilities to interfere with virus replication. Three tombusviruses tested were able to maintain both sat RNAs in N. benthamiana plants, although carnation Italian ringspot virus (CIRV) was a poor helper for sat RNA B1. Using chimeric viruses, a strong determinant for low sat RNA B1 accumulation was mapped to the 5'-terminal part of the genome of CIRV. The poor helper activity of CIRV was shown to be due to low sat RNA B1 replication. A single-nucleotide mutation in the start codon of CIRV ORF1 restored the ability to replicate sat RNA B1 to high levels. This mutant encodes an ORF1 that is 22 amino acids shorter at the N- terminus than the wild-type virus.

Original languageEnglish
Pages (from-to)129-138
Number of pages10
JournalVirology
Volume262
Issue number1
DOIs
Publication statusPublished - Sep 15 1999

Fingerprint

Tombusvirus
Satellite RNAs
Helper Viruses
RNA
Dianthus
Viruses
Viral RNA
Initiator Codon
Protoplasts
Virus Replication
Viral Load
Tobacco
Nucleotides
Complementary DNA
Clone Cells

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Interactions between tombusviruses and satellite RNAs of tomato bushy stunt virus : A defect in sat RNA B1 replication maps to ORF1 of a helper virus. / Célix, Ana; Burgyán, J.; Rodríguez-Cerezo, Emilio.

In: Virology, Vol. 262, No. 1, 15.09.1999, p. 129-138.

Research output: Contribution to journalArticle

@article{e724375fbd134cdaba033e74bc6617dc,
title = "Interactions between tombusviruses and satellite RNAs of tomato bushy stunt virus: A defect in sat RNA B1 replication maps to ORF1 of a helper virus",
abstract = "The biological properties of two recently described satellite RNAs of tomato bushy stunt virus (TBSV) were analyzed in natural and experimental hosts. Full-length cDNA clones were constructed for sat RNAs B1 (822 nt) and B10 (612 nt) and used in inoculations with satellite-free transcripts of different tombusviruses. In all virus-host combinations tested, TBSV sat RNA B10 drastically reduced the accumulation of viral genomic RNA and attenuated symptoms. In contrast, sat RNA B1 caused a less marked reduction of viral RNA level and did not have any effect on symptoms. Experiments with Nicotiana benthamiana protoplasts showed that the differential effects of sat RNAs B1 and B10 on TBSV titer were related to differential abilities to interfere with virus replication. Three tombusviruses tested were able to maintain both sat RNAs in N. benthamiana plants, although carnation Italian ringspot virus (CIRV) was a poor helper for sat RNA B1. Using chimeric viruses, a strong determinant for low sat RNA B1 accumulation was mapped to the 5'-terminal part of the genome of CIRV. The poor helper activity of CIRV was shown to be due to low sat RNA B1 replication. A single-nucleotide mutation in the start codon of CIRV ORF1 restored the ability to replicate sat RNA B1 to high levels. This mutant encodes an ORF1 that is 22 amino acids shorter at the N- terminus than the wild-type virus.",
author = "Ana C{\'e}lix and J. Burgy{\'a}n and Emilio Rodr{\'i}guez-Cerezo",
year = "1999",
month = "9",
day = "15",
doi = "10.1006/viro.1999.9865",
language = "English",
volume = "262",
pages = "129--138",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Interactions between tombusviruses and satellite RNAs of tomato bushy stunt virus

T2 - A defect in sat RNA B1 replication maps to ORF1 of a helper virus

AU - Célix, Ana

AU - Burgyán, J.

AU - Rodríguez-Cerezo, Emilio

PY - 1999/9/15

Y1 - 1999/9/15

N2 - The biological properties of two recently described satellite RNAs of tomato bushy stunt virus (TBSV) were analyzed in natural and experimental hosts. Full-length cDNA clones were constructed for sat RNAs B1 (822 nt) and B10 (612 nt) and used in inoculations with satellite-free transcripts of different tombusviruses. In all virus-host combinations tested, TBSV sat RNA B10 drastically reduced the accumulation of viral genomic RNA and attenuated symptoms. In contrast, sat RNA B1 caused a less marked reduction of viral RNA level and did not have any effect on symptoms. Experiments with Nicotiana benthamiana protoplasts showed that the differential effects of sat RNAs B1 and B10 on TBSV titer were related to differential abilities to interfere with virus replication. Three tombusviruses tested were able to maintain both sat RNAs in N. benthamiana plants, although carnation Italian ringspot virus (CIRV) was a poor helper for sat RNA B1. Using chimeric viruses, a strong determinant for low sat RNA B1 accumulation was mapped to the 5'-terminal part of the genome of CIRV. The poor helper activity of CIRV was shown to be due to low sat RNA B1 replication. A single-nucleotide mutation in the start codon of CIRV ORF1 restored the ability to replicate sat RNA B1 to high levels. This mutant encodes an ORF1 that is 22 amino acids shorter at the N- terminus than the wild-type virus.

AB - The biological properties of two recently described satellite RNAs of tomato bushy stunt virus (TBSV) were analyzed in natural and experimental hosts. Full-length cDNA clones were constructed for sat RNAs B1 (822 nt) and B10 (612 nt) and used in inoculations with satellite-free transcripts of different tombusviruses. In all virus-host combinations tested, TBSV sat RNA B10 drastically reduced the accumulation of viral genomic RNA and attenuated symptoms. In contrast, sat RNA B1 caused a less marked reduction of viral RNA level and did not have any effect on symptoms. Experiments with Nicotiana benthamiana protoplasts showed that the differential effects of sat RNAs B1 and B10 on TBSV titer were related to differential abilities to interfere with virus replication. Three tombusviruses tested were able to maintain both sat RNAs in N. benthamiana plants, although carnation Italian ringspot virus (CIRV) was a poor helper for sat RNA B1. Using chimeric viruses, a strong determinant for low sat RNA B1 accumulation was mapped to the 5'-terminal part of the genome of CIRV. The poor helper activity of CIRV was shown to be due to low sat RNA B1 replication. A single-nucleotide mutation in the start codon of CIRV ORF1 restored the ability to replicate sat RNA B1 to high levels. This mutant encodes an ORF1 that is 22 amino acids shorter at the N- terminus than the wild-type virus.

UR - http://www.scopus.com/inward/record.url?scp=0344199954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344199954&partnerID=8YFLogxK

U2 - 10.1006/viro.1999.9865

DO - 10.1006/viro.1999.9865

M3 - Article

C2 - 10489347

AN - SCOPUS:0344199954

VL - 262

SP - 129

EP - 138

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -