Interactions between the transcription and replication machineries regulate the RNA and DNA synthesis in the herpesviruses

Z. Boldogkői, Dóra Tombácz, Zsolt Balázs

Research output: Contribution to journalReview article

Abstract

The temporal coordination of viral gene expression is imperative for the regulation of the herpesvirus replication cycle. While the main factors of this transcriptional coordination are known, the subtler control mechanisms of gene expression remain elusive. Recent long read sequencing-based approached have revealed an intricate meshwork of overlaps between the herpesvirus transcripts and the overlap of the replication origins with noncoding RNAs. It has been shown that the transcriptional apparatuses can physically interfere with one another while transcribing overlapping regions. We hypothesize that transcriptional interference regulates the global gene expression across the herpesvirus genome. Additionally, an overall decrease in transcriptional activity in individual viral genes has been observed following the onset of DNA replication. An overlap of the replication origins with specific transcripts has also been described in several herpesviruses. The genome-wide interactions between the transcriptional apparatuses and between the replication and transcriptional machineries suggest the existence of novel layers of genetic regulation.

Original languageEnglish
JournalVirus Genes
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Herpesviridae
RNA
Replication Origin
Viral Genes
DNA
Gene Expression
Genome
Untranslated RNA
DNA Replication

Keywords

  • DNA replication
  • Herpesvirus
  • Nanopore sequencing
  • Transcriptional interference
  • Transcriptional overlap

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Interactions between the transcription and replication machineries regulate the RNA and DNA synthesis in the herpesviruses. / Boldogkői, Z.; Tombácz, Dóra; Balázs, Zsolt.

In: Virus Genes, 01.01.2019.

Research output: Contribution to journalReview article

@article{94068631a7a04fcbbf341536ef2e2f98,
title = "Interactions between the transcription and replication machineries regulate the RNA and DNA synthesis in the herpesviruses",
abstract = "The temporal coordination of viral gene expression is imperative for the regulation of the herpesvirus replication cycle. While the main factors of this transcriptional coordination are known, the subtler control mechanisms of gene expression remain elusive. Recent long read sequencing-based approached have revealed an intricate meshwork of overlaps between the herpesvirus transcripts and the overlap of the replication origins with noncoding RNAs. It has been shown that the transcriptional apparatuses can physically interfere with one another while transcribing overlapping regions. We hypothesize that transcriptional interference regulates the global gene expression across the herpesvirus genome. Additionally, an overall decrease in transcriptional activity in individual viral genes has been observed following the onset of DNA replication. An overlap of the replication origins with specific transcripts has also been described in several herpesviruses. The genome-wide interactions between the transcriptional apparatuses and between the replication and transcriptional machineries suggest the existence of novel layers of genetic regulation.",
keywords = "DNA replication, Herpesvirus, Nanopore sequencing, Transcriptional interference, Transcriptional overlap",
author = "Z. Boldogkői and D{\'o}ra Tomb{\'a}cz and Zsolt Bal{\'a}zs",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s11262-019-01643-5",
language = "English",
journal = "Virus Genes",
issn = "0920-8569",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Interactions between the transcription and replication machineries regulate the RNA and DNA synthesis in the herpesviruses

AU - Boldogkői, Z.

AU - Tombácz, Dóra

AU - Balázs, Zsolt

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The temporal coordination of viral gene expression is imperative for the regulation of the herpesvirus replication cycle. While the main factors of this transcriptional coordination are known, the subtler control mechanisms of gene expression remain elusive. Recent long read sequencing-based approached have revealed an intricate meshwork of overlaps between the herpesvirus transcripts and the overlap of the replication origins with noncoding RNAs. It has been shown that the transcriptional apparatuses can physically interfere with one another while transcribing overlapping regions. We hypothesize that transcriptional interference regulates the global gene expression across the herpesvirus genome. Additionally, an overall decrease in transcriptional activity in individual viral genes has been observed following the onset of DNA replication. An overlap of the replication origins with specific transcripts has also been described in several herpesviruses. The genome-wide interactions between the transcriptional apparatuses and between the replication and transcriptional machineries suggest the existence of novel layers of genetic regulation.

AB - The temporal coordination of viral gene expression is imperative for the regulation of the herpesvirus replication cycle. While the main factors of this transcriptional coordination are known, the subtler control mechanisms of gene expression remain elusive. Recent long read sequencing-based approached have revealed an intricate meshwork of overlaps between the herpesvirus transcripts and the overlap of the replication origins with noncoding RNAs. It has been shown that the transcriptional apparatuses can physically interfere with one another while transcribing overlapping regions. We hypothesize that transcriptional interference regulates the global gene expression across the herpesvirus genome. Additionally, an overall decrease in transcriptional activity in individual viral genes has been observed following the onset of DNA replication. An overlap of the replication origins with specific transcripts has also been described in several herpesviruses. The genome-wide interactions between the transcriptional apparatuses and between the replication and transcriptional machineries suggest the existence of novel layers of genetic regulation.

KW - DNA replication

KW - Herpesvirus

KW - Nanopore sequencing

KW - Transcriptional interference

KW - Transcriptional overlap

UR - http://www.scopus.com/inward/record.url?scp=85061566609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061566609&partnerID=8YFLogxK

U2 - 10.1007/s11262-019-01643-5

DO - 10.1007/s11262-019-01643-5

M3 - Review article

JO - Virus Genes

JF - Virus Genes

SN - 0920-8569

ER -