Interaction of Zinc-phthalocyanine-tetrasulphonate with different types of bacterial cells

F. Gabor, G. Csík, G. Rontó

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gram-negative Escherichia coli B. and Gram-positive Streptococcus faecalis were incubated with a water-soluble, second generation photosensitizer - Zn(II) - phthalocyanine - tetrasulphonate (ZnPcTS). The dark toxicity of the drug was checked at its different concentrations. Growth curves of treated and untreated cultures were assessed by turbidimetric measurement and compared by two-samples t-test. The quantity of incorporated phthalocyanine was determined by its fluorescence intensity and then correlated to the protein content of bacteria. ZnPcTS showed significantly greater uptake by Gram-positive S. faecalis than by Gram-negative E. coli B. The dye appears to interact with the two types of bacteria according to different pathways as suggested by the differences in temperature-dependence of the binding process. The amount of photosensitizer associated with the cells was also determined for different numbers of washing steps. The weakly bound fraction can be removed by two washing steps for both bacteria.

Original languageEnglish
Pages (from-to)294-298
Number of pages5
JournalMedical Science Monitor
Volume3
Issue number3
Publication statusPublished - 1997

Fingerprint

Photosensitizing Agents
Enterococcus faecalis
Bacteria
Escherichia coli
Drug-Related Side Effects and Adverse Reactions
Coloring Agents
Fluorescence
Temperature
Water
Growth
zinc tetrasulfophthalocyanine
Proteins
tetrasulfophthalocyanine
Zn(II)-phthalocyanine
phthalocyanine

Keywords

  • Cellular uptake
  • Dark toxicity
  • Gram-negative bacteria
  • Gram-positive bacteria
  • Photosensitization
  • Zinc-phthalocyanine-tetrasulphonate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Interaction of Zinc-phthalocyanine-tetrasulphonate with different types of bacterial cells. / Gabor, F.; Csík, G.; Rontó, G.

In: Medical Science Monitor, Vol. 3, No. 3, 1997, p. 294-298.

Research output: Contribution to journalArticle

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