Interaction of THP-1 monocytes with conidia and hyphae of different Curvularia strains

Eszter Judit Tóth, Éva Boros, Alexandra Hoffmann, Csilla Szebenyi, Mónika Homa, Gábor Nagy, C. Vágvölgyi, István Nagy, T. Papp

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Interaction of the human monocytic cell line, THP-1 with clinical isolates of three Curvularia species were examined. Members of this filamentous fungal genus can cause deep mycoses emerging in both immunocompromised and immunocompetent patients. It was found that monocytes reacted only to the hyphal form of Curvularia lunata. Cells attached to the germ tubes and hyphae and production of elevated levels of interleukin (IL)-8 and IL-10 and a low level of TNF-α were measured. At the same time, monocytes failed to produce IL-6. This monocytic response, especially with the induction of the anti-inflammatory IL-10, correlates well to the observation that C. lunata frequently cause chronic infections even in immunocompetent persons. Despite the attachment to the hyphae, monocytes could not reduce the viability of the fungus and the significant decrease in the relative transcript level of HLA-DRA assumes the lack of antigen presentation of the fungus by this cell type. C. spicifera and C. hawaiiensis failed to induce the gathering of the cells or the production of any analyzed cytokines. Monocytes did not recognize conidia of Curvularia species, even when melanin was lacking in their cell wall.

Original languageEnglish
JournalFrontiers in Immunology
Volume8
Issue numberOCT
DOIs
Publication statusPublished - Oct 18 2017

Fingerprint

Fungal Spores
Hyphae
Monocytes
Interleukin-10
HLA-DR alpha-Chains
Fungi
Mycoses
Melanins
Antigen Presentation
Immunocompromised Host
Interleukin-8
Cell Wall
Interleukin-6
Anti-Inflammatory Agents
Cytokines
Cell Line
Infection

Keywords

  • Curvularia
  • ELISA
  • Interleukin-10
  • Invasive mycosis
  • Melanin
  • Monocyte
  • Quantitative reverse transcription PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Interaction of THP-1 monocytes with conidia and hyphae of different Curvularia strains. / Tóth, Eszter Judit; Boros, Éva; Hoffmann, Alexandra; Szebenyi, Csilla; Homa, Mónika; Nagy, Gábor; Vágvölgyi, C.; Nagy, István; Papp, T.

In: Frontiers in Immunology, Vol. 8, No. OCT, 18.10.2017.

Research output: Contribution to journalArticle

Tóth, Eszter Judit ; Boros, Éva ; Hoffmann, Alexandra ; Szebenyi, Csilla ; Homa, Mónika ; Nagy, Gábor ; Vágvölgyi, C. ; Nagy, István ; Papp, T. / Interaction of THP-1 monocytes with conidia and hyphae of different Curvularia strains. In: Frontiers in Immunology. 2017 ; Vol. 8, No. OCT.
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