Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster.

A. H. Beaton, I. Kiss, D. Fristrom, J. W. Fristrom

Research output: Contribution to journalArticle

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Abstract

The 2B5 region on the X chromosome of Drosophila melanogaster forms an early ecdysone puff at the end of the third instar. The region is coextensive with a complex genetic locus, the Broad-Complex (BR-C). The BR-C is a regulatory gene that contains two major functional domains, the br domain and the l(1)2Bc domain. BR-C mutants prevent metamorphosis, including morphogenesis of imaginal discs; br mutants prevent elongation and eversion of appendages and l(1)2Bc mutants prevent fusion of the discs. The Stubble-stubbloid (Sb-sbd) locus at 89B9-10 is best known for the effects of its mutants on bristle structure. Mutants of the BR-C and the Sb-sbd locus interact to produce severe malformation of appendages. Viable heteroallelic and homoallelic combinations of Sb-sbd mutants, including loss-of-function mutants, affect the elongation of imaginal disc appendages. Thus, the Sb-sbd+ product is essential for normal appendage elongation. Sb-sbd mutants, however, do not affect eversion or fusion of discs. Correspondingly, only BR-C mutants deficient in br function interact with Sb-sbd mutants. The interaction occurs in deficiency heterozygotes using single, wild-type doses of the BR-C, of the Sb-sbd locus, or of both loci. These last results are formally consistent with the possibility that the BR-C acts as a positive regulator of the Sb-sbd locus. The data do not exclude other possible nonregulatory interactions between the two loci, e.g., interactions between the products of both genes.

Original languageEnglish
Pages (from-to)453-464
Number of pages12
JournalGenetics
Volume120
Issue number2
Publication statusPublished - Oct 1988

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Imaginal Discs
Drosophila melanogaster
Ecdysone
Genetic Loci
X Chromosome
Regulator Genes
Heterozygote
Morphogenesis
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Beaton, A. H., Kiss, I., Fristrom, D., & Fristrom, J. W. (1988). Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster. Genetics, 120(2), 453-464.

Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster. / Beaton, A. H.; Kiss, I.; Fristrom, D.; Fristrom, J. W.

In: Genetics, Vol. 120, No. 2, 10.1988, p. 453-464.

Research output: Contribution to journalArticle

Beaton, AH, Kiss, I, Fristrom, D & Fristrom, JW 1988, 'Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster.', Genetics, vol. 120, no. 2, pp. 453-464.
Beaton, A. H. ; Kiss, I. ; Fristrom, D. ; Fristrom, J. W. / Interaction of the Stubble-stubbloid locus and the Broad-complex of Drosophila melanogaster. In: Genetics. 1988 ; Vol. 120, No. 2. pp. 453-464.
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