Interaction of the antitumour drug 4-(9-acridinyiamino)-methanesulfon-m-anisidine.HCL (m-AMSA) with nucleic acids

F. Hudecz, J. Kajtár, M. Szekerke

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The interaction of AMSA with nucleic acids was studied by several techniques. Melting temperature and CD studies equally suggest that AMSA-binding is interfering with the secondary structure of DNA. An overlap by two mechanism of binding seems to exist. Based on the CD measurements at low drug concentration intercalation is the most likely way of binding. At higher drug concentration stacking interaction predominates leading to cooperativity and formation of oriented sheets of aromatic ring-systems as reflected in the optical activity induced in the metachromatic band of the achiral drug. No base-pair specificity could be confirmed; however, a high affinity of AMSA to poly(A) chains was demonstrated. The CD measurements did not indicate any significant interaction with RNA. The selectivity of the AMSA-DNA interaction can be regarded as an important argument in favour of the role of this interaction in the antitumour effect of the drug.

Original languageEnglish
Pages (from-to)6959-6974
Number of pages16
JournalNucleic Acids Research
Volume9
Issue number24
DOIs
Publication statusPublished - Dec 21 1981

Fingerprint

Amsacrine
Nucleic acids
Antineoplastic Agents
Nucleic Acids
Drugs
DNA
Intercalation
RNA
Interaction
Pharmaceutical Preparations
Melting point
Optical Rotation
Poly A
Optical Activity
Base Pairing
Freezing
Stacking
Secondary Structure
Selectivity
Melting

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Interaction of the antitumour drug 4-(9-acridinyiamino)-methanesulfon-m-anisidine.HCL (m-AMSA) with nucleic acids. / Hudecz, F.; Kajtár, J.; Szekerke, M.

In: Nucleic Acids Research, Vol. 9, No. 24, 21.12.1981, p. 6959-6974.

Research output: Contribution to journalArticle

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