Interaction of positively charged gold nanoparticles with cancer cells monitored by in situ label-free optical biosensor and transmission electron microscopy

Beatrix Peter, I. Lagzi, Satoshi Teraji, Hideyuki Nakanishi, L. Cervenák, Daniel Zambo, A. Deák, Kinga Molnár, Monika Truszka, Inna Székács, R. Horváth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Functionalized nanoparticles can penetrate into living cells and vesicles, opening up an extensive range of novel directions. For example, nanoparticles are intensively employed in targeted drug delivery and biomedical imaging. However, the real-time kinetics and dynamics of nanoparticle-living cell interactions remained uncovered. In this study, we in situ monitored the cellular uptake of gold nanoparticles - functionalized with positively charged alkaline thiol - into surface adhered cancer cells, by using a high-throughput label-free optical biosensor employing resonant waveguide gratings. The characteristic kinetic curves upon nanoparticle exposure of cell coated biosensor surfaces were recorded and compared to the kinetics of nanoparticle adsorption onto bare sensor surfaces. We demonstrated that from the above kinetic information one can conclude about the interactions between the living cells and nanoparticles. Real-time biosensor data suggested the cellular uptake of the functionalized nanoparticles by an active process. It was found that positively charged particles penetrate into the cells more effectively than negatively charged control particles, and the optimal size for cellular uptake of the positively charged particles is around 5 nm. These conclusions were obtained in a cost effective, fast and high-throughput manner. The fate of the nanoparticles was further revealed by electron microscopy on nanoparticle exposed and subsequently fixed cells, well confirming the results obtained by the biosensor. Moreover, ultrastructural study demonstrated the involvement of the endosomal-lysosomal system in the uptake of functionalized nanoparticles and suggested the type of the internalization pathway.

Original languageEnglish
JournalACS Applied Materials and Interfaces
DOIs
Publication statusAccepted/In press - Jan 27 2018

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Biosensors
Gold
Labels
Cells
Nanoparticles
Transmission electron microscopy
Kinetics
Charged particles
Throughput
Sulfhydryl Compounds
Electron microscopy
Waveguides
Imaging techniques
Adsorption
Sensors

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Interaction of positively charged gold nanoparticles with cancer cells monitored by in situ label-free optical biosensor and transmission electron microscopy. / Peter, Beatrix; Lagzi, I.; Teraji, Satoshi; Nakanishi, Hideyuki; Cervenák, L.; Zambo, Daniel; Deák, A.; Molnár, Kinga; Truszka, Monika; Székács, Inna; Horváth, R.

In: ACS Applied Materials and Interfaces, 27.01.2018.

Research output: Contribution to journalArticle

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AU - Teraji, Satoshi

AU - Nakanishi, Hideyuki

AU - Cervenák, L.

AU - Zambo, Daniel

AU - Deák, A.

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AU - Truszka, Monika

AU - Székács, Inna

AU - Horváth, R.

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