Interaction of mycotoxin zearalenone with human serum albumin

Miklós Poór, S. Kunsági-Máté, Mónika Bálint, C. Hetényi, Zsófia Gerner, Beáta Lemli

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Zearalenone (ZEN) is a mycotoxin produced mainly by Fusarium species. Fungal contamination of cereals and plants can result in the formation of ZEN, leading to its presence in different foods, animal feeds, and drinks. Because ZEN is an endocrine disruptor, it causes reproductive disorders in farm animals and hyperoestrogenic syndromes in humans. Despite toxicokinetic properties of ZEN were studied in more species, we have no information regarding the interaction of ZEN with serum albumin. Since albumin commonly plays an important role in the toxicokinetics of different toxins, interaction of ZEN with albumin has of high biological importance. Therefore the interaction of ZEN with human serum albumin (HSA) was investigated using spectroscopic methods, ultrafiltration, and molecular modeling studies. Fluorescence spectroscopic studies demonstrate that ZEN forms complex with HSA. Binding constant (K) of ZEN-HSA complex was quantified with fluorescence quenching technique. The determined binding constant (logK = 5.1) reflects the strong interaction of ZEN with albumin suggesting the potential biological importance of ZEN-HSA complex formation. Based on the results of the investigations with site markers as well as docking studies, ZEN occupies a non-conventional binding site on HSA. Considering the above listed observations, we should keep in mind this interaction if we would like to precisely understand the toxicokinetic behavior of ZEN.

Original languageEnglish
Pages (from-to)16-24
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume170
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Zearalenone
Mycotoxins
albumins
Serum Albumin
serums
interactions
Albumins
animals
fluorescence
Fluorescence
food
markers
Endocrine Disruptors
contamination
Domestic Animals
Fusarium
Ultrafiltration
quenching
disorders

Keywords

  • Albumin-ligand interaction
  • Fluorescence spectroscopy
  • Human serum albumin
  • Zearalenone

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

Interaction of mycotoxin zearalenone with human serum albumin. / Poór, Miklós; Kunsági-Máté, S.; Bálint, Mónika; Hetényi, C.; Gerner, Zsófia; Lemli, Beáta.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 170, 01.05.2017, p. 16-24.

Research output: Contribution to journalArticle

Poór, Miklós ; Kunsági-Máté, S. ; Bálint, Mónika ; Hetényi, C. ; Gerner, Zsófia ; Lemli, Beáta. / Interaction of mycotoxin zearalenone with human serum albumin. In: Journal of Photochemistry and Photobiology B: Biology. 2017 ; Vol. 170. pp. 16-24.
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