Interaction of mycotoxin alternariol with serum albumin

Eszter Fliszár-Nyúl, Beáta Lemli, Sándor Kunsági-Máté, Luca Dellafiora, Chiara Dall’Asta, Gabriele Cruciani, Gábor Pethő, Miklós Poór

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3 Citations (Scopus)

Abstract

Alternariol (AOH) is a mycotoxin produced by Alternaria species. In vitro studies suggest the genotoxic, mutagenic, and endocrine disruptor effects of AOH, and an increased incidence of esophageal cancer has been reported related to higher AOH exposure. Human serum albumin (HSA) is the most abundant plasma protein in the circulation, it is able to affect toxicokinetic properties of numerous xenobiotics. HSA forms stable complexes with several mycotoxins, however, the interaction of AOH with albumin has not been examined. In this study, the complex formation of AOH with HSA was tested, employing fluorescence spectroscopy, ultrafiltration, and molecular modeling. Each spectroscopic measurement shows the formation of stable AOH-HSA complexes (K = 4 × 105 L/mol). Investigations with site markers (in spectroscopic and ultrafiltration models) as well as modeling studies suggest that AOH occupies Sudlow’s site I as a high-affinity binding site in HSA. The binding affinity of AOH towards bovine, porcine, and rat albumins was also tested, suggesting that AOH binds to rat albumin with considerably higher affinity than other albumins tested. Our results demonstrate the strong interaction of AOH with serum albumins, suggesting the potential in vivo importance of these interactions.

Original languageEnglish
Article number2352
JournalInternational journal of molecular sciences
Volume20
Issue number9
DOIs
Publication statusPublished - May 1 2019

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Keywords

  • Albumin-ligandinteraction
  • Alternariol
  • Bindingsite
  • Fluorescence spectroscopy
  • Serumalbumin

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Fliszár-Nyúl, E., Lemli, B., Kunsági-Máté, S., Dellafiora, L., Dall’Asta, C., Cruciani, G., Pethő, G., & Poór, M. (2019). Interaction of mycotoxin alternariol with serum albumin. International journal of molecular sciences, 20(9), [2352]. https://doi.org/10.3390/ijms20092352