Interaction of fusarium mycotoxins, fusaproliferin and fumonisin B1, with DNA studied by electrospray ionization mass spectrometry

Gabriella Pocsfalvi, Alberto Ritieni, Giacomino Randazzo, András Dobó, Antonio Malorni

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Electrospray ionization mass spectrometry (ESI-MS) in negative ion mode was used to monitor the possible noncovalent adduct formations between DNA analogue oligonucleotides and two Fusarium mycotoxins, fumonisin B1 and fusaproliferin. Using mild experimental ESI conditions specific noncovalent interactions were detected between both single- and double-stranded model oligonucleotides and fusaproliferin with 1:1 stoichiometry. Similar association complexes were observed for the deacetyl derivative of fusaproliferin. There were no peaks due to adduct formation present in the mass spectra of fumonisin B1, incubated with oligonucleotides in a wide concentration range, suggesting no specific interaction for this molecule. In a competitive complexation reaction, another mycotoxin, the beauvericin, forms more stable association complex with DNA than fusaproliferin. These findings can be of use in the understanding of molecular mechanisms of action during apoptosis and can be correlated with the teratogenic effect of fusaproliferin.

Original languageEnglish
Pages (from-to)5795-5801
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume48
Issue number12
DOIs
Publication statusPublished - Dec 1 2000

Keywords

  • Beauvericin
  • ESI-MS
  • Fumonisin B
  • Fusaproliferin
  • Mycotoxins
  • Noncovalent interaction
  • Oligonucleotides

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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