Interaction of differentiated human adipocytes with macrophages leads to trogocytosis and selective IL-6 secretion

A. K. Sárvári, Q. M. Doan-Xuan, Z. Bacsó, I. Csomós, Z. Balajthy, L. Fésüs

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Obesity leads to adipose tissue inflammation that is characterized by increased release of proinflammatory molecules and the recruitment of activated immune cells. Although macrophages are present in the highest number among the immune cells in obese adipose tissue, not much is known about their direct interaction with adipocytes.We have introduced an ex vivo experimental system to characterize the cellular interactions and the profile of secreted cytokines in cocultures of macrophages and human adipocytes differentiated from either mesenchymal stem cells or a preadipocyte cell line. As observed by time-lapse microscopy, flow, and laserscanning cytometry, macrophages phagocytosed bites of adipocytes (trogocytosis), which led to their de novo, phagocytosis and Nf-kB-dependent synthesis, then release of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1. IL-6 secretion was not accompanied by secretion of other proinflammatory cytokines, such as tumor necrosis factor (TNf)-α and IL-8, except MCP-1. LPSinduced release of TNf-α, IL-8 and MCP-1 was decreased in the presence of the differentiated adipocytes but the IL-6 level did not subside suggesting that phagocytosis-dependent IL-6 secretion may have significant regulatory function in the inflamed adipose tissue.

Original languageEnglish
Article numbere1613
JournalCell Death and Disease
Volume6
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

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Adipocytes
Chemokine CCL2
Interleukin-6
Macrophages
Phagocytosis
Adipose Tissue
Interleukin-8
Tumor Necrosis Factor-alpha
Cytokines
Bites and Stings
Coculture Techniques
Mesenchymal Stromal Cells
Microscopy
Flow Cytometry
Obesity
Inflammation
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Cancer Research
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Interaction of differentiated human adipocytes with macrophages leads to trogocytosis and selective IL-6 secretion. / Sárvári, A. K.; Doan-Xuan, Q. M.; Bacsó, Z.; Csomós, I.; Balajthy, Z.; Fésüs, L.

In: Cell Death and Disease, Vol. 6, No. 1, e1613, 01.01.2015.

Research output: Contribution to journalArticle

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