Interaction of cytocidal drugs and the inhibition of caspase-3 by 3- nitrosobenzamide

Rudolf Mihalik, Páll Bauer, István Peták, Péter Krajcsi, Attila Marton, Kun Ernest, László Kopper

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7 Citations (Scopus)


The effect of 3-nitrosobenzamide (NOBA) on the etoposide, staurosporine and dexamethason induced rapid (4-6 hr), caspase-dependent apoptosis was investigated in thymocytes and lymphoma cells by flow cytometric assay of DNA fragmentation. When NOBA (ED50 = 4 μM) was added to these cell systems, the rapid onset of apoptosis was prevented. Such apparent protection by NOBA was related to the inactivation of caspase-3, by s-nitrosylation of 1.3 mol - SH per enzyme molecule out of 7 -SH groups. Since NOBA by itself induces DNA fragmentation within 18 hr in lymphoma cells, our results indicate that at least two active cell death pathways exist with apparent dissimilar kinetics and molecular mechanisms.

Original languageEnglish
Pages (from-to)875-879
Number of pages5
JournalInternational Journal of Cancer
Issue number6
Publication statusPublished - Aug 23 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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