Interaction of citrinin with human serum albumin

Miklós Poór, Beáta Lemli, Mónika Bálint, C. Hetényi, Nikolett Sali, T. Kőszegi, S. Kunsági-Máté

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Citrinin (CIT) is a mycotoxin produced by several Aspergillus, Penicillium, and Monascus species. CIT occurs worldwide in different foods and drinks and causes health problems for humans and animals. Human serum albumin (HSA) is the most abundant plasma protein in human circulation. Albumin forms stable complexes with many drugs and xenobiotics; therefore, HSA commonly plays important role in the pharmacokinetics or toxicokinetics of numerous compounds. However, the interaction of CIT with HSA is poorly characterized yet. In this study, the complex formation of CIT with HSA was investigated using fluorescence spectroscopy and ultrafiltration techniques. For the deeper understanding of the interaction, thermodynamic, and molecular modeling studies were performed as well. Our results suggest that CIT forms stable complex with HSA (logK ~ 5.3) and its primary binding site is located in subdomain IIA (Sudlow’s Site I). In vitro cell experiments also recommend that CIT-HSA interaction may have biological relevance. Finally, the complex formations of CIT with bovine, porcine, and rat serum albumin were investigated, in order to test the potential species differences of CIT-albumin interactions.

Original languageEnglish
Pages (from-to)5155-5166
Number of pages12
JournalToxins
Volume7
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Citrinin
Serum Albumin
Albumins
Monascus
Molecular modeling
Pharmacokinetics
Mycotoxins
Aspergillus
Penicillium
Fluorescence Spectrometry
Fluorescence spectroscopy
Ultrafiltration
Xenobiotics
Medical problems
Thermodynamics
Rats
Blood Proteins
Animals
Swine
Binding Sites

Keywords

  • Citrinin
  • Fluorescence spectroscopy
  • Human serum albumin
  • Species differences
  • Ultrafiltration

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Interaction of citrinin with human serum albumin. / Poór, Miklós; Lemli, Beáta; Bálint, Mónika; Hetényi, C.; Sali, Nikolett; Kőszegi, T.; Kunsági-Máté, S.

In: Toxins, Vol. 7, No. 12, 01.12.2015, p. 5155-5166.

Research output: Contribution to journalArticle

Poór, Miklós ; Lemli, Beáta ; Bálint, Mónika ; Hetényi, C. ; Sali, Nikolett ; Kőszegi, T. ; Kunsági-Máté, S. / Interaction of citrinin with human serum albumin. In: Toxins. 2015 ; Vol. 7, No. 12. pp. 5155-5166.
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