interaction of antithrombin III and thrombinantithrombin III complex with cultured aortic endothelial cells

Katalin Bartha, T. Kovács, Istvan Lerant, Bella Papp, Eva Csonka, K. Kolev, R. Machovich

Research output: Contribution to journalArticle

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Abstract

The binding of antithrombin III, thrombin, thrombin-antithrombin III complex to endothelial cells was investigated. While the rate of the binding of thrombin to these cells was very rapid, that of antithrombin III was relatively slow and the thrombin-antithrombin III complex was intermediate. Binding kinetics indicated that antithrombin III, like thrombin, showed high affinity to endothelial cells; with a Kd of 3×10-8M and with 5×104 binding sites per cell. The dissociation of the inhibitor molecule was also rapid, i.e., approximately 70% bound antithrombin III was released in 2 minutes. Heparin, in a 100-fold molar excess to antithrombin III, or the modification of lysine residues of the inhibitor involved in the interaction with heparin, did not influence the association of antithrombin III with endothelial cells. In addition, antithrombin III did not compete with thrombin blocked in its active center for binding to endothelial cells. It is suggested that the binding sites of endothelial cells are different for thrombin and antithrombin III, and antithrombin III does not bind to these cells through its heparin binding domain.

Original languageEnglish
Pages (from-to)541-552
Number of pages12
JournalThrombosis Research
Volume47
Issue number5
DOIs
Publication statusPublished - Sep 1 1987

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Antithrombin III
Endothelial Cells
Thrombin
Heparin
Binding Sites
Lysine

Keywords

  • antithrombin III
  • Endothelial cells
  • thrombin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

interaction of antithrombin III and thrombinantithrombin III complex with cultured aortic endothelial cells. / Bartha, Katalin; Kovács, T.; Lerant, Istvan; Papp, Bella; Csonka, Eva; Kolev, K.; Machovich, R.

In: Thrombosis Research, Vol. 47, No. 5, 01.09.1987, p. 541-552.

Research output: Contribution to journalArticle

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