The interaction of amino acids with 14 commercial anticancer drugs was studied by charge-transfer reversed-phase thin-layer chromatotography and the retention matrix was evaluated by principal component analysis. The majority of amino acids had a negligible effect on the retention of anticancer drugs. Only dicarboxy amino acids and Trp exerted a considerable impact on the retention of drugs, indicating the involvement of hydrophilic forces (probably hydrogen bond formation) and stacking interactions in the binding of drugs to amino acids. Stepwise regression analysis proved that both the pI value of amino acids and the pK value of the amino acid side chain significantly influence the strength of interaction. The findings support the hypothesis that the binding of anticancer drugs to proteins may involve both hydrophilic forces occurring between the corresponding polar substructures of drugs and amino acids and stacking interactions between the various ring structures. The relative importance of these interactions depends on the character of the amino acid residue and the chemical structure of drugs.
|Number of pages||11|
|Journal||Biochemistry and Molecular Biology International|
|Publication status||Published - Jan 1 1994|
ASJC Scopus subject areas
- Molecular Biology