Interaction of amino acids and glutathione with the fungicide 1-phenyl-2-nitro-3-acetoxyprop-1-ene studied by charge-transfer chromatography

G. Gullner, T. Cserháti, Attila Kis-Tamás, Gyula Mikite

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Reversed-phase charge-transfer thin-layer chromatography has been applied to study the interaction of the fungicide 1-phenyl-2-nitro-3-acetoxyprop-1-ene with amino acids and glutathione. The fungicide interacts preferentially with thiol amino acids and glutathione, but weak interactions with other amino acids were also observed. Our results confirm that the fungicide can act by blocking the sulfhydryl groups of essential proteins in fungi. The interaction with thiol amino acids involves molecular complex formation, but that with glutathione is of covalent character. The pH value has no significant effect on the interaction.

Original languageEnglish
Pages (from-to)211-220
Number of pages10
JournalJournal of Chromatography A
Volume355
Issue numberC
DOIs
Publication statusPublished - 1986

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Fungicides
Chromatography
Glutathione
Charge transfer
Amino Acids
Sulfhydryl Compounds
Thin layer chromatography
Thin Layer Chromatography
Fungi
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Interaction of amino acids and glutathione with the fungicide 1-phenyl-2-nitro-3-acetoxyprop-1-ene studied by charge-transfer chromatography. / Gullner, G.; Cserháti, T.; Kis-Tamás, Attila; Mikite, Gyula.

In: Journal of Chromatography A, Vol. 355, No. C, 1986, p. 211-220.

Research output: Contribution to journalArticle

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