Interaction between various resistance modifiers and apoptosis inducer 12H-benzo[a]phenothiazine

I. Mucsi, Andreas Varga, Masami Kawase, Noboru Motohashi, J. Molnár

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effect of some resistance modifiers on apoptosis induction by a benzo[a]phenothiazine derivative was studied on the L5178Y mouse lymphoma cells (parent) and its multidrug resistant (MDR) subline. For evaluation of apoptosis the cells were stained with FITC-labelled annexin V and propidium iodide and the results were analysed by flow cytometry. 12H-benzo[a]phenothiazine [M627] induced apoptosis both in the parent cells and in the MDR cells. The apoptosis induction by [M627] was not affected significantly by post- or pre-treatment with resistance modifiers, while in the cells treated by (±)-verapamil before and after apoptosis induction with [M627], the apoptosis was somewhat higher. The resistance modifier compounds alone also induced apoptosis and it was slightly higher in the parent cells than its MDR1/A gene-transformed subline.

Original languageEnglish
Pages (from-to)2833-2836
Number of pages4
JournalAnticancer Research
Volume22
Issue number5
Publication statusPublished - Sep 2002

Fingerprint

Apoptosis
Propidium
Fluorescein-5-isothiocyanate
Annexin A5
Verapamil
benzophenothiazine
Lymphoma
Flow Cytometry
Genes

Keywords

  • 12H-benzo[α]phenothiazine
  • Apoptosis induction
  • Mouse T cell lymphoma cells
  • Resistance modifiers
  • Trifluoroketones

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Interaction between various resistance modifiers and apoptosis inducer 12H-benzo[a]phenothiazine. / Mucsi, I.; Varga, Andreas; Kawase, Masami; Motohashi, Noboru; Molnár, J.

In: Anticancer Research, Vol. 22, No. 5, 09.2002, p. 2833-2836.

Research output: Contribution to journalArticle

Mucsi, I. ; Varga, Andreas ; Kawase, Masami ; Motohashi, Noboru ; Molnár, J. / Interaction between various resistance modifiers and apoptosis inducer 12H-benzo[a]phenothiazine. In: Anticancer Research. 2002 ; Vol. 22, No. 5. pp. 2833-2836.
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