Interaction between the pyruvate dehydrogenase complex and citrate synthase

B. Sümegi, László Gyócsi, István Alkonyi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Kinetic studies of the individual reaction of pig heart pyruvate dehydrogenase complex (pyruvate dehydrogenase (pyruvate:lipoamide oxidoreductase (decarboxylating and acceptor-acetylating), EC 1.2.4.1); dihydrolipoamide reductase(NAD+) (NADH:lipoamide oxidoreductase, EC 1.6.4.3); dihydrolipo-amide acetyltransferase (acetyl-CoA;dihydrolipoamide S-acetyltransferase, EC 2.3.1.12)), citrate synthase (citrate oxaloacetate-lyase (pro-3S-CH2COO- → acetyl-CoA), EC 4.1.3.7) and the pyruvate dehydrogenase complex-citrate synthase coupled system show that the KmCoA value of pyruvate dehydrogenase complex and KmCoASAc value of citrate synthase decrease in the coupled system when compared to those in the individual enzyme reactions. The explanation for this interaction may be an association between the two enzymes. When it was centrifuged with 150 000 × g for 140 min, 30% of the citrate synthase sedimented in the presence of the pyruvate dehydrogenase complex, while no sedimentation was observed in the absence of the pyruvate dehydrogenase complex. Sedimentation of cytoplasmic malate dehydroganase, phosphotransacetylase, hemoglobin and Blue albumin were negligible under the same condition. In gel chromatography experiments a significant peak of citrate synthase activity co-migrated with the pyruvate dehydrogenase complex peak. This observation also suggests the possible association of two enzymes.

Original languageEnglish
Pages (from-to)158-166
Number of pages9
JournalBBA - Enzymology
Volume616
Issue number2
DOIs
Publication statusPublished - Dec 4 1980

Fingerprint

Pyruvate Dehydrogenase Complex
Citrate (si)-Synthase
Oxidoreductases
NAD
Phosphate Acetyltransferase
Pyruvate Synthase
Enzymes
Dihydrolipoyllysine-Residue Acetyltransferase
Acetyltransferases
Pyruvic Acid
Amides
Gel Chromatography
Albumins
Hemoglobins
Swine

Keywords

  • (Pig heart mitochondria)
  • Citrate synthase
  • Protein:protein interaction
  • Pyruvate dehydrogenase complex

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Interaction between the pyruvate dehydrogenase complex and citrate synthase. / Sümegi, B.; Gyócsi, László; Alkonyi, István.

In: BBA - Enzymology, Vol. 616, No. 2, 04.12.1980, p. 158-166.

Research output: Contribution to journalArticle

Sümegi, B. ; Gyócsi, László ; Alkonyi, István. / Interaction between the pyruvate dehydrogenase complex and citrate synthase. In: BBA - Enzymology. 1980 ; Vol. 616, No. 2. pp. 158-166.
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