Interaction between NAD-dependent isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase complex, and NADH: ubiquinone oxidoreductase.

Z. Porpaczy, B. Sümegi, I. Alkonyi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Interaction between the alpha-ketoglutarate dehydrogenase complex and NAD+-dependent isocitrate dehydrogenase was detected with a variety of techniques including polyethylene glycol precipitation, ultracentrifugation, and centrifugal gel filtration on a Sepharose 6B column. The interaction was specific in that citrate synthase, cytosolic malate dehydrogenase, and NADP-dependent isocitrate dehydrogenase did not interact with alpha-ketoglutarate dehydrogenase complex. The interaction was not inhibited by either 0.1 M KCl or 0.4 M (NH4)2SO4, but was completely prevented by 5% glycerol. A new method for the preparation of NADH: ubiquinone oxidoreductase resulted in an enzyme having a protein subunit composition similar to that of classical complex I preparation. Evidence is given for the existence of ternary complexes containing NADH:ubiquinone oxidoreductase-alpha-ketoglutarate dehydrogenase complex-NAD-dependent isocitrate dehydrogenase and NADH: ubiquinone oxidoreductase-alpha-ketoglutarate dehydrogenase complex-succinate thiokinase. These data suggest that a part of the citric acid cycle may be located in the vicinity of NADH: ubiquinone oxidoreductase. These complexes may facilitate the transport of metabolites among these enzymes without their equilibrating with the whole compartment.

Original languageEnglish
Pages (from-to)9509-9514
Number of pages6
JournalJournal of Biological Chemistry
Volume262
Issue number20
Publication statusPublished - Jul 15 1987

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Ketoglutarate Dehydrogenase Complex
Electron Transport Complex I
Isocitrate Dehydrogenase
NAD
Malate Dehydrogenase (NADP+)
Citrate (si)-Synthase
Citric Acid Cycle
Ultracentrifugation
Protein Subunits
Succinic Acid
Enzymes
Metabolites
Sepharose
Glycerol
Gel Chromatography
Gels
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Interaction between NAD-dependent isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase complex, and NADH : ubiquinone oxidoreductase. / Porpaczy, Z.; Sümegi, B.; Alkonyi, I.

In: Journal of Biological Chemistry, Vol. 262, No. 20, 15.07.1987, p. 9509-9514.

Research output: Contribution to journalArticle

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