Interactions of neutral polymers (PVA and PVP) with liposomal membranes were investigated, and based on the adsorption isotherms we have found out that PVA adsorbs in higher rate than PVP due to the stronger H-bridge bonds. The polymer-membrane interactions were detected by several measuring techniques and among them the dynamic laser light scattering convinced us that in case of stabilizing liposomes with PVA the average diameter of liposomes is larger, thus the layer adsorbed is thicker than when they are interacted with PVP. Titration microcalorimetric measurements were only made on the systems containing PVA proving the existence of exothermic interactions at the beginning of the adsorption process. XRD studies on films made of liposomes and swollen by exposure to water vapour proved the structure-modifying effect of the polymer present in the membrane. PVA allows smaller expansion to the membrane bilayer than PVP via swelling with water vapour. From remote loading experiments we have concluded that our test compound, acridine orange (marked as NA in the text) can be entrapped in liposomes most effectively in media buffered with Tris-HCl. The optimal NA/PL ratio was found to be 0.1. Drug release experiments also confirmed the stabilizing effect of the polymer.
|Translated title of the contribution||Interaction between liposomes and neural polymers; effect of stabilizing on drug release|
|Number of pages||11|
|Journal||Magyar Kemiai Folyoirat, Kemiai Kozlemenyek|
|Publication status||Published - Nov 1 2001|
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