Interaction between hypothalamic peptides in a superfused pituitary cell system

Sandor Vigh, Andrew V. Schally

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Recently two hypothalamic releasing factors have been isolated, sequenced and synthesized: corticotropin-releasing factor (CRF) from sheep and rat hypothalami; and growth hormone-releasing factor (GH-RF) from tumors in human pancreas (hpGH-RF) and from rat hypothalami (rhGH-RF). Their biological potencies were tested by various laboratories in vivo and in vitro using rat pituitary cell cultures and the pituitary quarters method. In the present study, we investigated the dynamics of the release of pituitary hormones and the interaction between CRF and hGH-RF and several brain peptides in a pituitary cell-superfusion system. A dose-related ACTH release was found when the cells were superfused with different doses of synthetic CRF. Synthetic hGH-RF44, hGH-RF40, hGH-RF1-29 and purified porcine hypothalamic GH-RF caused similar dose-related releases of GH. In this system, we demonstrated interactions between CRF and vasopressin, CRF and SP, hGH-RF and vasopressin and hGH-RF and PHI-27. We conclude that the control of pituitary hormone secretion is a complex process; several factors may interact with each releasing or inhibiting factor to modulate their effects.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalPeptides
Volume5
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 1984

Fingerprint

Corticotropin-Releasing Hormone
Peptides
Rats
Growth Hormone-Releasing Hormone
Pituitary Hormones
Vasopressins
Hypothalamus
Hypothalamic Hormones
Pituitary Hormone-Releasing Hormones
Cell culture
Adrenocorticotropic Hormone
Tumors
Pancreas
Brain
Sheep
Swine
Cell Culture Techniques
Neoplasms

Keywords

  • CRF
  • GH-RF
  • Interaction
  • PHI-27
  • Somatostatin
  • Substance P
  • Superfused pituitary cell-column system
  • Vasopressin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Interaction between hypothalamic peptides in a superfused pituitary cell system. / Vigh, Sandor; Schally, Andrew V.

In: Peptides, Vol. 5, No. SUPPL. 1, 1984, p. 241-247.

Research output: Contribution to journalArticle

Vigh, Sandor ; Schally, Andrew V. / Interaction between hypothalamic peptides in a superfused pituitary cell system. In: Peptides. 1984 ; Vol. 5, No. SUPPL. 1. pp. 241-247.
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